Figure 1. ELISA using WU polyomavirus (WUPyV) viral protein 1 (VP1) or KI polyomavirus (KIPyV) VP1 as the target antigen. A) Coomassie blue staining of a sodium dodecyl sulfate–polyacrylamide gel that contains bacterially expressed glutathione S-transferase (GST)–KIPyV VP1 and GST–WUPyV VP1 before and after glutathione-affinity purification. B) ELISA using rabbit hyperimmune serum and human WU polyomavirus convalescent-phase serum preincubated with buffer alone, GST protein, or GST–WUPyV VP1. Error bars indicate mean and SD.
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