Susceptibilities of Nonhuman Primates to Chronic Wasting Disease
Figure 3. Immunohistochemical analysis of squirrel monkeys infected with chronic wasting disease (CWD) agent. Panels A, C, and E–M are from squirrel monkeys infected with CWD. Panels B and D are from an uninfected monkey showing no pathologic changes or positive staining for protease-resistant prion protein (PrPres). Panels A and B, cerebral cortex stained with hematoxylin and eosin; panels C and D, thalamus stained with antibody 3F4 against PrP (arrows); panels E and F, cerebellar granular cell layer and spinal cord, respectively, stained with antibody 3F4; panel G, gray matter within the internal capsule stained with antibody D13 against PrP; panel H, corpus callosum (right) stained with antibody D13 showing more intense staining than the adjacent cortex (left); panel I, frontal cortex (fc), claustrum (cl), and caudate (ca) stained with antibody 3F4 (abundant vacuoles in the putamen [arrows]); panels J–M, lymphatic tissue stained with antibody 3F4; panels J and K, PrPres staining in spleen of monkey 322; panels L and M, PrPres-positive mesenteric lymph node from orally infected monkey 301. Rectangles in panels J and L show areas enlarged in panels K and M, respectively. Antibodies D13 and 3F4 showed similar results for each monkey regarding the distribution, characteristics, and plaque size of PrPres. Scale bars: panels A–I, 50 μm; panel J, 100 μm; panels K and M, 25 μm; panel L, 250 μm.