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Volume 15, Number 9—September 2009

Dispatch

Monitoring of Putative Vectors of Bluetongue Virus Serotype 8, Germany

Bernd Hoffmann, Burkhard Bauer, Christian Bauer, Hans-Joachim Bätza, Martin Beer, Peter-Henning Clausen, Martin Geier, Jörn M. Gethmann, Ellen Kiel, Gabriele Liebisch, Arndt Liebisch, Heinz Mehlhorn, Günter A. Schaub, Doreen Werner, and Franz J. ConrathsComments to Author 
Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (B. Hoffmann, M. Beer); Free University of Berlin, Berlin, Germany (B. Bauer, P.-H. Clausen); Justus Liebig University Giessen, Giessen, Germany (C. Bauer); Federal Ministry for Food, Agriculture and Consumer Protection, Bonn, Germany (H.-J. Bätza); University of Regensburg, Regensburg, Germany (M. Geier); Friedrich-Loeffler-Institut, Wusterhausen, Germany (J.M. Gethmann, F.J. Conraths); Carl von Ossietzky University Oldenburg, Oldenburg, Germany (E. Kiel); Zecklab, Burgwedel, Germany (G. Liebisch, A. Liebisch); Heinrich-Heine-University of Düsseldorf, Düsseldorf, Germany (H. Mehlhorn); Ruhr University, Bochum, Germany (G.A. Schaub); Leibniz-Center for Agricultural Landscape Research e. V, Müncheberg, Germany (D. Werner)

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Table 1

Genetic characterization of batches of midges of the Culicoides obsoletus group, Germany, April 2007–May 2008*

Batch no. rRT-PCR, Ct C. obsoletus sensu stricto C. scoticus C. chiopterus C. dewulfi
270/38F 20.10 +++ +++ +++
276/2T 20.74 +++ +++ +++
306/12FN 21.33 +++ +++ +++
304/34B 21.60 +++ +++
276/2O 23.40 +++ +++ +++
296/52Q 23.87 +++ +++ +++
296/44G 24.00 +++ +++ +++
296/22R 25.90 +++ +++ +++
296/52AL 27.26 +++ +++ +++
306/1X 27.30 +++
306/1A 27.60 +++
306/1E 27.70 +++
306/1Z 27.70 +++
270/45B 27.76 +++ +++
306/1L 27.90 +++
306/1T 27.90 +++
263/46B 28.00 +++ ++ +++
306/1H 28.00 +++
306/1AA 28.00 +++
306/1S 28.10 +++
306/1AC 28.10 +++
18/156B 28.18 +++ + +++
346/29C 28.19 +++ +
306/1R 28.30 +++
306/1V 28.30 +++ +++
306/1N 28.40 +++ ++
276/10S 28.41 +++ +++ +++
306/1I 28.50 +++
306/1M 28.70 +++ + +++
263/46A 28.80 +++ +++
296/51AH 28.82 +++ ++ +++ +++
306/1D 28.90 +++
171/13E 29.00 +++ +++
306/1C 29.00 +++ +++
304/1H 29.10 +++ +++ +++
306/1B 29.20 +++
306/1F 29.20 +++
306/1U 29.20 +++
336/24B 29.21 +++ +++
306/12LR 29.26 +++ +++ +++
306/1K 29.30 +++
306/1J 29.40 +++
270/55I 29.47 +++ +++ +++

*rRT-PCR, real-time reverse transcription–PCR; Ct, cycle threshold; –, no visible band; +, faint; ++, distinct; +++, strong band after agarose gel electrophoresis of PCR products and staining with ethidium bromide.

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