Parvovirus 4 in Blood Donors, France
Figure. Alignment of parvovirus 4 (PARV4) sequences showing the location of the 2 probes used in the real-time experiments. A) Partial sequences used for the design of probe PARV4-N: PARV4 prototype isolate (AY622943) and in-house PCR products characterized initially (GenBank accession nos. FJ883557–61). B) Examples of point mutations located on the PARV4-O recognition site identified in amplicons originating from samples positive with PARV4-N assay. Mismatches identified in the alignments are underlined. Nucleotides shown in lowercase letters correspond to 5′/3′ ends of the real-time primers. Mismatches identified in the alignments are underlined and in boldface.