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Volume 16, Number 11—November 2010

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Genome Sequence Conservation of Hendra Virus Isolates during Spillover to Horses, Australia

Glenn A. MarshComments to Author , Shawn Todd, Adam Foord, Eric Hansson, Kelly Davies, Lynda Wright, Chris Morrissy, Kim Halpin1, Deborah Middleton, Hume E. Field, Peter Daniels, and Lin-Fa Wang
Author affiliations: Australian Animal Health Laboratory, Geelong, Victoria, Australia (G.A. Marsh, S. Todd, A. Foord, E. Hansson, K. Davies, L. Wright, C. Morrissy, K. Halpin, D. Middleton, P. Daniels, L.-F. Wang); Australian Biosecurity Cooperative Research Centre for Emerging Infectious Diseases, Brisbane, Queensland, Australia (H.E. Field, L.-F. Wang); Biosecurity Queensland, Brisbane (H.E. Field); 1Current affiliation: Life Technologies, Singapore.

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Figure

Phylogenetic trees based on the N open reading frame (ORF) (A, B) and the G ORF (C, D), with DNA sequences used for A and C and amino acid sequences for B and D. All sequences were compared with the reference sequences for each of the known henipavirus strains; Hendra virus/Australia/horse/1994/Hendra (GenBank accession no. AF017149), Nipah virus/Malaysia/human/1999 (GenBank accession no. AF212302), Nipah virus/Bangladesh/human/2004/Rajbari, R1 (GenBank accession no. AY988601), Nipah virus/Cambo

Figure. Phylogenetic trees based on the N open reading frame (ORF) (A, B) and the G ORF (C, D), with DNA sequences used for A and C and amino acid sequences for B and D. All sequences were compared with the reference sequences for each of the known henipavirus strains; Hendra virus/Australia/horse/1994/Hendra (GenBank accession no. AF017149), Nipah virus/Malaysia/human/1999 (GenBank accession no. AF212302), Nipah virus/Bangladesh/human/2004/Rajbari, R1 (GenBank accession no. AY988601), Nipah virus/Cambodia/bat/2004/KHM/CSUR381 (GenBank accession no. AY858110 [N ORF] and AY858111 [G ORF]) and Nipah virus/India/human/2007/GF (GenBank accession no. FJ513078). Phylogenetic trees were constructed by using the neighbor-joining algorithm in the MEGA4 software package (15). Scale bars represent substitutions per site. HeV, Hendra virus; NiV, Nipah virus.

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