Figure 4. Effects of temperature on cyprinid herpesvirus 3 replication in Cyprinus carpio carp brain cells. After infection, cells were kept at 22°C (A) or shifted to 30°C (B–D); some cells were returned to 22°C at 24 hours (C) or 48 hours (D) postinfection. Uninfected control cells (E) and infected cells at 9 days postinfection were fixed, stained, and photographed. Viral replication was highest in cells maintained at 22°C and lowest in those maintained at 30°C. Original magnification ×20. Adapted with permission from Dishon et al. (18).