Figure 5. Skin of carp as a portal of entry for cyprinid herpesvirus 3. A schematic representation of the system used to restrict viral inoculation to the fish skin is shown on the left. The lower drawing shows the conditions under which 6 fish were inoculated by restricted contact of the virus with the skin located posterior to the anterior part of the dorsal fin. The upper drawing shows control conditions under which 6 fish were inoculated in the system but without the latex diaphragm dividing the fish body into 2 isolated parts, enabling virus to reach the entire fish body. The fish were infected by bathing them for 24 h in water containing 2 × 103 PFU/mL of a recombinant cyprinid herpesvirus 3 strain able to emit bioluminescence. All fish were analyzed 24 h postinfection (hpi) by bioluminescence imaging. After an additional incubation period of 24 h in individual tanks containing fresh water, they were reanalyzed by bioluminescence imaging at 48 hpi. Three representative fish are shown. The images are shown with standardized minimum and maximum threshold values for photon flux. Adapted with permission from Costes et al. (29).
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