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Volume 16, Number 12—December 2010

Research

Eastern Equine Encephalitis Virus in Mosquitoes and Their Role as Bridge Vectors

Philip M. ArmstrongComments to Author  and Theodore G. Andreadis
Author affiliations: Author affiliation: The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA

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Table

Eastern equine encephalitis virus isolated and virus titers from mosquitoes obtained in Connecticut, USA, 2009*

Mosquito species No. mosquitoes collected No. virus isolates Infection rate/1,000 mosquitoes, MLE (95% CI) Mean Ct by qRT-PCR Mean titer
log10 PFU/ mosquito pool % Mosquito pools >3.0 log10 PFU/ mosquito pool
Aedes cinereus 15,294 6 0.4 (0.2–0.8) 34.0 2.92 16.7
Ae. vexans 26,462 2 0.1 (0–0.2) 33.0 1.15 0
Anopheles punctipennis 5,573 4 0.7 (0.2–1.7) 34.6 1.69 0
An. quadrimaculatus 607 2 3.3 (0.6–10.6) 35.9 1.67 0
An. walkeri 2,381 2 0.8 (0.2–2.7) 35.1 1.43 0
Culex restuans 14,609 1 0.1 (0–0.3) 35.2 0.85 0
Cx. salinarius 12,605 3 0.2 (0.1–0.6) 32.1 1.31 0
Culiseta melanura 25,595 83 3.4 (2.8–4.2) 22.3 6.55 88.0
Ochlerotatus canadensis 40,543 10 0.2 (0.1–0.4) 32.9 2.82 10.0
Oc. cantator 4,457 1 0.2 (0–1.1) 31.8 1.54 0
Oc. triseriatus 3,000 1 0.3 (0–1.6) >50 1.60 0
Oc. trivittatus 23,340 2 0.1 (0–0.3) 38.8 <0.8 0
Psorophora ferox 13,677 1 0.1 (0–0.35) 34.0 <0.8 0
Uranotaenia sapphirina 2,954 4 1.4 (0.4–3.2) 36.5 1.00 0
Remaining species† 100,544 0

*MLE, maximum-likelihood estimation; CI, confidence interval; Ct, cycle threshold; qRT-PCR, quantitative reverse transcription–PCR; –, not applicable. Mean Ct and PFU values were calculated for mosquito pools positive by qRT-PCR and plaque titration.
†n = 21.

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