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Volume 16, Number 12—December 2010

Research

Reassortant Group A Rotavirus from Straw-colored Fruit Bat (Eidolon helvum)

Mathew D. Esona, Slavica Mijatovic-Rustempasic, Christina Conrardy, Suxiang Tong, Ivan V. Kuzmin, Bernard Agwanda, Robert F. Breiman, Krisztian Banyai, Michael Niezgoda, Charles E. Rupprecht, Jon R. Gentsch, and Michael D. BowenComments to Author 
Author affiliations: Author affilations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.D. Esona, S. Mijatovic-Rustempasic, C. Conrardy, S. Tong , I.V. Kuzmin, M. Niezgoda, C.E. Rupprecht, J.R. Gentsch, M.D. Bowen); National Museum of Kenya, Nairobi, Kenya (B. Agwanda); Centers for Disease Control and Prevention–Kenya, Nairobi (R.F. Breiman); Hungarian Academy of Sciences, Budapest, Hungary (K. Banyai)

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Table

Newly designed primers used for amplification and sequencing of bat rotavirus genes*

Primer Sequence, 5′ → 3′ Gene Nucleotide position, strand
VP1_Bat GCT TCG AAT GGA GAA TCG CG VP1 596–576, –
VP1_Bat CTC CTG GTG TGT ACC TAC C VP1 485–504, +
VP1_Bat CCT CGT GTG TAA ATA CGG ACA VP1 1130–1151, +
VP2_Bat CAG AGC AGG CTA AGA AGC AGA CTA VP2 393–370, –
VP2_Bat GCA GCA CCA ATT TGG GTT GAG VP2 810–830, +
VP2_Bat CTC AAC CCA AAT TGG TGC TG VP2 830–810, –
VP2_Bat CTG AAT CTG AGC TTC AGT TG VP2 1206–1187, –
VP2_Bat GAT GTA GCT AGA GTG CCA G VP2 2247–2265, +
VP2_Bat CTG GCA CTC TAG CTA CAT C VP2 2265–2247, –
VP2_Bat GGT GGC GAA TTA TGA TTG G VP2 2723–2741, +
VP6_Bat TCG GTC TAT GGA ATG TGA AAC CTG TTC VP6 380–354, –
VP7_Bat CAG ATG TCG TCG ATA ATG VP7 693–710, +
VP7_Bat TGG CGT ACG CAG TGT CCA TTG AC VP7 218–196, –
VP7_Bat CGA GTT GGC GTA CGC AGT G VP7 258–240, –
NSP2_Bat CAA CTT CCA CAT TGT AAA CGC NSP2 574–555, –
NSP2_Bat CTT TCG CGA ATA CAC TGG T NSP2 1087–1068, –
NSP2_Bat CT GAT AGA GTG TAT GCG AC NSP2 727–747, +
NSP3_Bat CCT CAC TCT CAT CTT TCG GGT CTT C NSP3 401–376, –
NSP3_Bat GGA GTT ACC GAG TGA AGC GAA GG NSP3 673–695, +
NSP5_Bat ACG CCA GCA TCT GCA TTT GTC NSP5 279–258, –

*VP, viral protein; NSP, nonstructural protein.

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