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Volume 16, Number 3—March 2010

Dispatch

Putative New Lineage of West Nile Virus, Spain

Ana VázquezComments to Author , María Paz Sánchez-Seco, Santiago Ruiz, Francisca Molero, Lourdes Hernández, Juana Moreno, Antonio Magallanes, Concepción Gómez Tejedor, and Antonio Tenorio
Author affiliations: Centro Nacional de Microbiología–Instituto de Salud Carlos III, Majadahonda, Spain (A. Vázquez, M.P. Sánchez-Seco, F. Molero, L. Hernández, A. Tenorio); Diputación Provincial de Huelva, Huelva, Spain (S. Ruiz, J. Moreno, A. Magallanes); Laboratorio Central de Veterinaria, Algete, Spain (C. Gómez Tejedor)

Main Article

Figure

Phylogenetic tree of 79 WNV isolates by the neighbor-joining method and distance-p model on MEGA3.1 (www.megasoftware.net/mega_dos.html). Bootstrap values correspond to 1,000 replications. A) Analysis of a 1,813-nt fragment of the nonstructural protein 5 (NS5) gene. B) Analysis of the 800-nt fragment of the NS5 gene. KOUV (strain DakArD1470, AF013384) and Malaysia (strain KUN MP502–66, GU047874) (boldface) were also used to obtain this tree. Scale bars indicate nucleotide substitutions per site.

Figure. Phylogenetic tree of 79 WNV isolates by the neighbor-joining method and distance-p model on MEGA3.1 (www.megasoftware.net/mega_dos.html). Bootstrap values correspond to 1,000 replications. A) Analysis of a 1,813-nt fragment of the nonstructural protein 5 (NS5) gene. B) Analysis of the 800-nt fragment of the NS5 gene. KOUV (strain DakArD1470, AF013384) and Malaysia (strain KUN MP502–66, GU047874) (boldface) were also used to obtain this tree. Scale bars indicate nucleotide substitutions per site. WNV, West Nile virus; nt, nucleotide; MBV, mosquito-borne viruses; JEG, Japanese encephalitis group; JEV, Japanese encephalitis virus; USUV, Usutu virus. Viruses used in the phylogenetic study (GenBank accession nos.): WNV lineage 1 (AY712948, AY712947, AY490240, AY278442, AY278441, AY277252, AF404757, AF404756, AF404755, AF404754, AF404753, AF481864, AY603654, AY646354, AY289214, AY795965, AY842931, AY660002, AF196835, DQ164206, DQ164202, DQ164197, AF260969, AF260968, AF260967, DQ211652, DQ164204, DQ164200, DQ164201, AF533540, DQ005530, DQ118127, DQ164205, DQ164203, DQ164199, D00246, DQ164196, DQ080058, DQ080054, DQ080055, DQ080056, DQ164193, DQ164186, DQ164195, DQ164191, DQ164205, DQ080053, AY848696, AB185917, DQ080052, AB185914, DQ080051, DQ164189, AF404756, DQ164190, AY712945, AY712946, DQ080059, DQ164188, DQ164187, DQ164192, AF404757, AY277252, AY274505); WNV lineage 2 (DQ116961, DQ318019, M12294, EF429200, AY532665, EF429198, EF429199, EF429197, NC001563, AY688948, DQ176636, DQ318020); WNV lineage 3 (AY765264); WNV lineage 4 (AY277251); WNV lineage 5 (DQ256376); Spanish WNV (HU2925/06, GU047875); JEV: (NC001437); and USUV (NC006551) (as an outgroup).

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