Use of Avian Bornavirus Isolates to Induce Proventricular Dilatation Disease in Conures
Figure 2. A) Avian bornavirus (ABV)–infected duck embryonic fibroblast (DEF) cell culture 6 days after injection with hindbrain tissues from an African gray parrot with confirmed proventricular dilatation disease (AG5) and staining by an indirect immunofluorescence assay for ABV N-protein. Speckled immunofluorescence is typical of bornavirus infection. Original magnification ×40. B) DEFs 3 days after injection with forebrain from a yellow-collared macaw with confirmed proventricular dilatation disease (M24). Nuclear and cytoplasmic fluorescence in DEFs stained by immunofluorescence assay for ABV N-protein. Original magnification ×40.