TY - JOUR AU - Gray, Patricia AU - Hoppes, Sharman AU - Suchodolski, Paulette AU - Mirhosseini, Negin AU - Payne, Susan AU - Villanueva, Itamar AU - Shivaprasad, H.L AU - Honkavuori, Kirsi S. AU - Briese, Thomas AU - Reddy, Sanjay M. AU - Tizard, Ian T1 - Use of Avian Bornavirus Isolates to Induce Proventricular Dilatation Disease in Conures T2 - Emerging Infectious Disease journal PY - 2010 VL - 16 IS - 3 SP - 473 SN - 1080-6059 AB - Avian bornavirus (ABV) is a newly discovered member of the family Bornaviridae that has been associated with the development of a lethal neurologic syndrome in birds, termed proventricular dilatation disease (PDD). We successfully isolated and characterized ABV from the brains of 8 birds with confirmed PDD. One isolate was passed 6 times in duck embryo fibroblasts, and the infected cells were then injected intramuscularly into 2 healthy Patagonian conures (Cyanoliseus patagonis). Clinical PDD developed in both birds by 66 days postinfection. PDD was confirmed by necropsy and histopathologic examination. Reverse transcription–PCR showed that the inoculated ABV was in the brains of the 2 infected birds. A control bird that received uninfected tissue culture cells remained healthy until it was euthanized at 77 days. Necropsy and histopathologic examinations showed no abnormalities; PCR did not indicate ABV in its brain tissues. KW - Avian bornavirus KW - viral encephalitis KW - proventriculus KW - parrots KW - viruses KW - research DO - 10.3201/eid1603.091257 UR - https://wwwnc.cdc.gov/eid/article/16/3/09-1257_article ER - End of Reference