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Volume 16, Number 4—April 2010

Dispatch

Novel Corynebacterium diphtheriae in Domestic Cats

Aron J. HallComments to Author , Pamela K. Cassiday, Kathryn A. Bernard, Frances Bolt, Arnold G. Steigerwalt, Danae Bixler, Lucia C. Pawloski, Anne M. Whitney, Masaaki Iwaki, Adam Baldwin, Christopher G. Dowson, Takako Komiya, Motohide Takahashi, Hans P. Hinrikson, and Maria L. Tondella
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A.J. Hall, P.K. Cassiday, A.G. Steigerwalt, L.C. Pawloski, A.M. Whitney, H.P. Henrikson, M.L. Tondella); West Virginia Department of Health and Human Resources, Charleston, West Virginia, USA (A.J. Hall, D. Bixler); Public Health Agency of Canada, Winnipeg, Manitoba, Canada (K.A. Bernard); University of Warwick, Coventry, UK (F. Bolt, A. Baldwin, C.G. Dowson); National Institute of Infectious Diseases, Tokyo, Japan (M. Iwaki, T. Komiya, M. Takahashi)

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Figure 1

Neighbor-joining phylogenetic tree based on 16S rRNA gene sequence analysis of Corynebacterium diphtheriae isolates, including 4 feline isolates from West Virginia, 2008 (ATCC BAA-1774, CD 448, CD 449, CD 450). The tree was constructed from a 1,437-bp alignment of 16S rRNA gene sequences by using the neighbor-joining method and Kimura 2-parameter substitution model. Bootstrap values (expressed as percentages of 1,000 replicates) >40% are illustrated at branch points. Feline isolates had 100%

Figure 1. Neighbor-joining phylogenetic tree based on 16S rRNA gene sequence analysis of Corynebacterium diphtheriae isolates, including 4 feline isolates from West Virginia, 2008 (ATCC BAA-1774, CD 448, CD 449, CD 450). The tree was constructed from a 1,437-bp alignment of 16S rRNA gene sequences by using the neighbor-joining method and Kimura 2-parameter substitution model. Bootstrap values (expressed as percentages of 1,000 replicates) >40% are illustrated at branch points. Feline isolates had 100% identity with each other and >99.1% identity with C. diphtheriae biotypes gravis and belfanti. GenBank accession nos. given in parentheses. ATCC, American Type Culture Collection; CD, Centers for Disease Control and Prevention identifier number; NCTC, National Collection of Type Cultures. Scale bar indicates number of substitutions per site.

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