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Volume 16, Number 4—April 2010

Research

Livestock-associated Methicillin-Resistant Staphylococcus aureus Sequence Type 398 in Humans, Canada

George R. Golding, Louis Bryden, Paul N. Levett, Ryan R. McDonald, Alice Wong, John Wylie, Morag R. Graham, Shaun Tyler, Gary Van Domselaar, Andrew E. Simor, Denise Gravel, and Michael R. MulveyComments to Author 
Author affiliations: National Microbiology Laboratory, Winnipeg, Manitoba, Canada (G.R. Golding, L. Bryden, M.R. Graham, S. Tyler, G. Van Domselaar, M.R. Mulvey); Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada (P.N. Levett, R.R. McDonald); Royal University Hospital, Saskatoon, Saskatchewan, Canada (A. Wong); Cadham Provincial Laboratories, Winnipeg (J. Wylie); Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada (A.E. Simor); Public Health Agency of Canada, Ottawa, Ontario, Canada (D. Gravel)

Main Article

Table 3

Open reading frames of the novel staphylococcal cassette chromosome mecV subtype in methicillin-resistant Staphylococcus aureus isolate 08 BA 02176, from woman in Canada, 2008*

ORF Location, bp† Predicted gene size, bp Gene‡ Product description Amino acid identity, %§ GenBank accession no.
Sk01 1–480 480 orfX Conserved hypothetical protein 100 gb|ACC96139.1|
Sk02 609–1595 987 None ADP-ribosylglycohydrolase 99 gb|AAW53059.1|
Sk03 1614–2948 1335 None Permease for cytosine/purines; uracil; thiamine; allantoin 98 gb|AAW53058.1|
Sk04 2999–3883 885 None Ribokinase 98 gb|AAW53057.1|
Sk05 (4013–4687) 675 tnp Transposase for IS431 100 dbj|BAD24823.1|
Sk06 4945–5112 168 None HMG-CoA synthase truncation 100 ref|YP_184940.1|
Sk07 6029–6772 744 ugpQ Glycerophosphoryl diester phosphodiesterase 100 ref|NP_370563.1|
Sk08 6869–7297 429 maoC Hypothetical protein 100 ref|YP_184943.1|
Sk09 (7343–9349) 2007 mecA Penicillin-binding protein 2′ 100 dbj|BAG06200.1|
Sk10 9449–9559 Unknown ψmecR1 Truncated signal transducer protein MecR1 100 ref|YP_252007.1|
Sk11 9597–9740 144 ψtnp Partial transposase for insertion sequence–like element IS431mec 100 dbj|BAH57698.1|
Sk12 (10331–10759) 429 None Hypothetical protein 100 dbj|BAD24829.1|
Sk13 10840–11769 930 None Hypothetical protein 100 gb|ACL99839.1|
Sk14 11931–13919 1989 None Hypothetical protein 100 gb|ACL99840.1|
Sk15 14114–15223 1110 None Hypothetical protein 100 gb|ACL99841.1|
Sk16 15584–17200 1617 None Hypothetical protein 100 gb|ACL99843.1|
Sk17 17425–19104 1680 ccrC Cassette chromosome recombinase C 100 gb|ACL99844.1|
Sk18 19193–19531 339 None Hypothetical protein 100 gb|ACL99845.1|
Sk19 19625–19936 312 None Hypothetical protein 100 gb|ACL99846.1|
Sk20 19951–20454 504 None Hypothetical protein 100 gb|ACL99847.1|
Sk21 20469–20690 222 None Hypothetical protein 100 gb|ACL99848.1|
Sk22 (20853–21256) 403 ψhsdR Truncated hsdR 92 dbj|BAG71456.1|
Sk23 22888–23793 906 cas1 CRISPR–associated Cas1 family protein 91 gb|AAW53332.1|
Sk24 23793–24098 306 cas2 CRISPR-associated protein Cas2 87 gb|AAW53331.1|
Sk25 24112–26385 2274 csm1 CRISPR-associated protein; Csm1 family 92 gb|AAW53330.1|
Sk26 26388–26813 426 csm2 CRISPR-system related protein 94 gb|AAW53329.1|
Sk27 26815–27459 645 csm3 CRISPR-associated RAMP protein 96 gb|AAW53328.1|
Sk28 27530–28378 849 csm4 CRISPR-associated RAMP protein 91 gb|AAW53327.1|
Sk29 28381–29403 1023 csm5 CRISPR-associated Csm5 family protein 92 gb|AAW53326.1|
Sk30 29403–30671 1269 csm6 CRISPR-associated protein (Cas_Csm6) 73 gb|AAW53325.1|
Sk31 30668–31402 735 cas6 CRISPR-associated protein C 86 gb|AAW53324.1|

*ORF, open reading frame; CRISPR, clustered regularly interspaced short palindromic repeats.
†Parentheses indicate complement sequences.
‡None indicates no name given.
§Comparisons of translated query versus protein databases was determined by using BLASTX 2.2.21 (www.ncbi.nlm.nih.gov/blast/Blast.cgi).

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