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Volume 16, Number 5—May 2010

Research

Historical Distribution and Molecular Diversity of Bacillus anthracis, Kazakhstan

Alim M. Aikembayev1, Larissa Lukhnova, Gulnara Temiraliyeva, Tatyana Meka-Mechenko, Yerlan Pazylov, Sarkis Zakaryan, Georgiy Denissov, W. Ryan Easterday, Matthew N. Van Ert2, Paul Keim, Stephen C. Francesconi, Jason K. Blackburn3, Martin Hugh-JonesComments to Author , and Ted Hadfield
Author affiliations: Kazakhstan Scientific Center for Quarantine and Zoonotic Diseases, Almaty, Kazakhstan (A.M. Aikembayev, L. Lukhnova, G. Temiraliyeva, T. Meka-Mechenko, Y. Pazylov, S. Zakaryan, G. Denissov); Northern Arizona University, Flagstaff, Arizona, USA (W.R. Easterday, P. Keim); Midwest Research Institute, Palm Bay, Florida, USA (M.N. Van Ert, T. Hadfield); The Translational Genomics Research Institute, Phoenix, Arizona, USA (P. Keim); Naval Medical Research Center, Silver Spring, Maryland, USA (S.C. Francesconi); California State University, Fullerton, California, USA (J.K. Blackburn); Louisiana State University, Baton Rouge, Louisiana, USA (M. Hugh-Jones);

Main Article

Table 3

Variable number tandem repeat sizes for Bacillus anthracis isolates, Kazakhstan*

Kazakhstan genotype no. MLVA group† MLVA genotype vrrA vrrB1 vrrB2 vrrC1 vrrC2 CG-3 pX01 pX02
1 A1.a Gt-13 (4) 313 229 162 613 604 153 132 137
2 A1.a Novel 313 229 162 613 604 153 135 137
3 A1.a Novel 313 229 162 613 604 153 129 139
4 A1.a Novel 313 229 162 613 604 153 129 137
5 A1.a Novel 313 229 162 613 604 153 138 137
6 A1.a Gt-6 (4) 301 229 162 613 604 153 126 137
7 A1.a Gt-3 (4) 313 229 162 613 604 153 126 137
8 A1.a Novel 313 229 162 613 604 153 132 139
9 Novel Novel 325 229 162 613 604 158 132 137
10 A4 Novel 313 229 162 538 604 158 126 137
11 Novel Novel 313 229 162 583 532 153 129 141
12 A3b Novel 313 229 162 583 532 158 126 139

*Raw allele sizes were determined by electrophoresis on the ABI 310 (Applied Biosystems, Inc., Foster City, CA, USA); sizes were compared to control variable number tandem repeats and corrected to the sizes reported by Keim et al. (4).
†MLVA, multilocus variable number tandem repeat. MLVA group determined by unweighted pair group method arithmetic mean clustering with the diverse 89 genotypes described by Keim et al. (4).

Main Article

1Current affiliation: Republican Sanitary Epidemiologic Station, Almaty, Kasakhstan.

2Current affiliation: VEN Consulting, LLC, Melbourne, Florida, USA.

3Current affiliation: University of Florida, Gainesville, Florida, USA.

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