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Volume 16, Number 9—September 2010

Research

Worldwide Diversity of Klebsiella pneumoniae That Produce β-Lactamase blaKPC-2 Gene1

Gaëlle Cuzon, Thierry NaasComments to Author , HaVy Truong, Maria-Virginia Villegas, Karin T. Wisell, Yehuda Carmeli, Ana. C. Gales, Shiri Navon-Venezia, John P. Quinn, and Patrice Nordmann
Author affiliations: Author affiliations: Institut National de la Santé et de la Recherche Médicale, Paris, France (G. Cuzon, T. Naas, H. Truong, P. Nordmann); International Center for Medical Research and Training, Cali, Colombia (M.V. Villegas); Swedish Institute for Infectious Disease Control, Stockholm, Sweden (K.T. Wisell); Sourasky Medical Center, Tel Aviv, Israel (Y. Carmeli, S. Navon-Venezia); Universidade Federal de São Paulo, São Paulo, Brazil (A.C. Gales); John H. Stroger Jr. Hospital of Cook County, Chicago, Illinois, USA (J.P. Quinn); Chicago Infectious Disease Research Institute, Chicago (J.P. Quinn)

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Figure 2

A) Plasmid extractions of culture of clinical Klebsiella pneumoniae isolates that produce β-lactamase blaKPC-2 gene. B) Southern hybridization of transferred plasmid extraction, conducted with an internal probe for blaKPC-2. Lane 1, K. pneumoniae YC (11); lane 2, K. pneumoniae GR (21); lane 3, K. pneumoniae K271 (25); lane 4, K. pneumoniae KN2303 (13); lane 5, K. pneumoniae KN633 (13); lane 6, K. pneumoniae INC H1521-6; lane 7, K. pneumoniae INC H1516-6; lane 8, K. pneumoniae HPTU 27635; lane 9,

Figure 2. A) Plasmid extractions of culture of clinical Klebsiella pneumoniae isolates that produce β-lactamase blaKPC-2 gene. B) Southern hybridization of transferred plasmid extraction, conducted with an internal probe for blaKPC-2. Lane 1, K. pneumoniae YC (11); lane 2, K. pneumoniae GR (21); lane 3, K. pneumoniae K271 (25); lane 4, K. pneumoniae KN2303 (13); lane 5, K. pneumoniae KN633 (13); lane 6, K. pneumoniae INC H1521-6; lane 7, K. pneumoniae INC H1516-6; lane 8, K. pneumoniae HPTU 27635; lane 9, K. pneumoniae HPTU 2020532; lane 10, K. pneumoniae A28006 (16); lane 11, K. pneumoniae A28008 (16); lane 12, K. pneumoniae A28009 (16); lane 13, K. pneumoniae A28011 (16); lane 14, K. pneumoniae A33504 (16); lane 15, K. pneumoniae 475; lane 16, K. pneumoniae 588; and lane 17, Escherichia coli 50192 harboring 4 plasmids (7, 48, 66, and 154 kb).

Main Article

1This study was presented in part at the Interscience Conference on Antimicrobial Agents and Chemotherapy; 2009 Sep 12–15; San Francisco, CA, USA.

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