Volume 16, Number 9—September 2010
Research
Worldwide Diversity of Klebsiella pneumoniae That Produce β-Lactamase blaKPC-2 Gene1
Gaëlle Cuzon, Thierry Naas
, HaVy Truong, Maria-Virginia Villegas, Karin T. Wisell, Yehuda Carmeli, Ana. C. Gales, Shiri Navon-Venezia, John P. Quinn, and Patrice Nordmann
, HaVy Truong, Maria-Virginia Villegas, Karin T. Wisell, Yehuda Carmeli, Ana. C. Gales, Shiri Navon-Venezia, John P. Quinn, and Patrice NordmannTable 2
Primers used for PCR of Klebsiella pneumoniae isolates producing β-lactamase blaKPC-2 gene*
| Primer name | Primer no.† | Sequence, 5′ → 3′ |
|---|---|---|
| KpcA | 1 | CTGTCTTGTCTCTCATGGCC |
| KpcB | 2 | CCTCGCTGTGCTTGTCATCC |
| 4281 | 3 | GGCACGGCAAATGACTA |
| 4714 | 4 | GAAGATGCCAAGGTCAATGC |
| EcoRIout | 5 | CACCCGACCTGGACGAACTA |
| 3′YCEnd | 6 | GCATCAAACGGAAGCAAAAG |
| 3781L | 7 | CACAGCGGCAGCAAGAAAGC |
| 3098U | 8 | TGACCCTGAGCGGCGAAAGC |
| 905L | 9 | GCGACCGGTCAGTTCCTTCT |
| 816U | 10 | CACCTACACCACGACGAACC |
| 141R-6 | 11 | TCACCGGCCCTCACCTTTGG |
| 5′endYC | 12 | CTTAGCAAATGTGGTGAACG |
| Pre-SHV-5 U | – | GGTCAGCGCGAGAAGCATCC |
| Pre-SHV-5 L | – | AAATAGCGTTCATCGTCAAT |
| Pre-TEM 1 | – | GTATCCGCTCATGAGACAATA |
| Pre-TEM 2 | – | TCTAAAGTATATATGAGTAAACTTGGTCTG |
| OXA-9 A | – | TTCGTTTCCGCCACTCTCCC |
| OXA-9 B | – | ACGAGAATATCCTCTCGTGC |
| CTX-M A | – | CGCTTTGCGATGTGTCAG |
| CTX-M B | – | ACC GCG ATA TCG TTG GT |
1This study was presented in part at the Interscience Conference on Antimicrobial Agents and Chemotherapy; 2009 Sep 12–15; San Francisco, CA, USA.
