Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options
CDC Home

Volume 16, Number 9—September 2010

Dispatch

Novel Hepatitis E Virus Genotype in Norway Rats, Germany

Reimar Johne, Gerald Heckel, Anita Plenge-Bönig, Eveline Kindler, Christina Maresch, Jochen Reetz, Anika Schielke, and Rainer G. UlrichComments to Author 
Author affiliations: Author affiliations: Federal Institute for Risk Assessment, Berlin, Germany (R. Johne, J. Reetz, A. Schielke); University of Bern, Bern, Switzerland (G. Heckel, E. Kindler); Swiss Institute of Bioinformatics, Lausanne, Switzerland (G. Heckel); Institute of Hygiene and Environment Hamburg, Hamburg, Germany (A. Plenge-Bönig); Friedrich-Loeffler-Institut, Greifswald–Insel Riems, Germany (C. Maresch, R.G. Ulrich); Free University of Berlin, Berlin (A. Schielke)

Main Article

Figure 2

Immunohistochemical staining (peroxidase-antiperoxidase (PAP) technique) of liver samples from 2 rat-hepatitis E virus (HEV)–positive Norway rats from Germany, July 2009. Arrows indicate immunohistochemical positive reactions in the cytoplasm of single hepatocytes (A) and in a few foci in hepatocytes and stellate cells (B). For PAP staining, deparaffinized slides of liver samples were incubated with anti-HEV–positive human serum, which had been previously used to detect rat HEV by using solid ph

Figure 2. Immunohistochemical staining (peroxidase-antiperoxidase (PAP) technique) of liver samples from 2 rat-hepatitis E virus (HEV)–positive Norway rats from Germany, July 2009. Arrows indicate immunohistochemical positive reactions in the cytoplasm of single hepatocytes (A) and in a few foci in hepatocytes and stellate cells (B). For PAP staining, deparaffinized slides of liver samples were incubated with anti-HEV–positive human serum, which had been previously used to detect rat HEV by using solid phase immunoelectron microscopy (10), for 1 h at 37°C with protein A (Sigma-Aldrich, Steinheim, Germany) at a dilution of 1:100 for 45 min at 37°C and finally with PAP complexes from rabbits (Sigma, St. Louis, MO, USA) at a dilution 1:200 for 45 min at 37°C. AEC (3-amino-9-ethylcarbazol; Sigma Chemie GmbH, Deisenhofen, Germany) was used as the substrate chromogene. The slides were counterstained with hematoxylin and subsequently analyzed by light microscopy. Scale bars = 20 µm.

Main Article

Top of Page

 

Past Issues

Select a Past Issue:

Art in Science - Selections from Emerging Infectious Diseases
Now available for order



CDC 24/7 – Saving Lives, Protecting People, Saving Money. Learn More About How CDC Works For You…

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO