Novel Hepatitis E Virus Genotype in Norway Rats, Germany
Reimar Johne, Gerald Heckel, Anita Plenge-Bönig, Eveline Kindler, Christina Maresch, Jochen Reetz, Anika Schielke, and Rainer G. Ulrich
Author affiliations: Author affiliations: Federal Institute for Risk Assessment, Berlin, Germany (R. Johne, J. Reetz, A. Schielke); University of Bern, Bern, Switzerland (G. Heckel, E. Kindler); Swiss Institute of Bioinformatics, Lausanne, Switzerland (G. Heckel); Institute of Hygiene and Environment Hamburg, Hamburg, Germany (A. Plenge-Bönig); Friedrich-Loeffler-Institut, Greifswald–Insel Riems, Germany (C. Maresch, R.G. Ulrich); Free University of Berlin, Berlin (A. Schielke)
Figure A1. Results of real-time reverse transcription–PCR showing cycle threshold (Ct) values of viral load in different tissues of Norway rats nos. 63 (white bars) and 68 (gray bars). For RNA isolation, 10 mg of tissue or 10 µL of blood were homogenized and used. The PCR was selective for a region in the open reading frame 2 of rat hepatitis E virus (rHEV) and was based on primers rHEV-forward (5′-TACCCGATGCCGGGCAGT-3′) and rHEV-reverse (5′-ATCCACATCTGGGACAGG-3′) and probe (5′-6FAM-AATGACAGCACAGGCACC-BBQ-3′). Error bars indicate SD. *Brain sample from rat no. 63 contained blood.
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