Volume 16, Number 9—September 2010
Analysis of Avian Hepatitis E Virus from Chickens, China
Avian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported. We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.
Hepatitis E virus (HEV) is a nonenveloped, positive-sense, single-stranded RNA virus. It has 3 open reading frames (ORFs) and a genome size of 7.2 kb (1). So far, HEV strains are classified into 4 major genotypes, and genotypes 3 and 4 are probably zoonotic.
Avian HEVs have been identified from chickens with big liver syndrome and hepatitis–splenomegaly syndrome. Each syndrome mainly causes increased deaths, reduced egg production, and enlarged liver and spleen (2); hepatitis–splenomegaly syndrome also causes accumulation of bloody fluid in the abdomen and vasculitis and amyloidosis in the liver (3,4). Molecular epidemiologic investigations have shown that avian HEV infection in chickens is endemic to the United States and Spain (5,6). Because propagating avian HEV in cell culture or embryonated eggs is difficult (2,3), avian HEV is primarily detected by reverse transcription–PCR (RT-PCR). However, only 4 complete or near-complete genomic sequences have been reported to GenBank (7–9). We identified and analyzed the near-complete genomic sequence of avian HEV in a chicken flock from the People’s Republic of China.
In May 2009, hepatitis–splenomegaly syndrome affected a flock of 37-week-old broiler breeder hens in Shandong, China. This flock had a history of decreased egg production. Affected chickens had regressive ovaries, extensive necrosis and hemorrhage of the liver, and enlarged liver and spleen. Antibodies against avian HEV ORF2 were detected in 80 of 94 serum samples from the same chicken flock, according to ELISA (5,10) with the truncated ORF2 protein used by Guo et al (10) and chicken serum diluted 1:100 in 0.5% Tween-20 phosphate-buffered saline containing 2.5% nonfat dry milk and 10% Escherichia coli lysate. On the basis of previous results, we used a cutoff optical density of 0.43 (11). Using a published method (12), we detected an avian HEV ORF2 RNA gene with 242 bp in 7 of 10 fecal and 5 of 8 bile samples.
From the bile samples that were positive for the avian HEV ORF2 gene, we used nested RT-PCR with 5 overlapping fragments to amplify the near-complete genomic sequence of avian HEV. Primers were designed on the basis of the other 4 avian HEV near-complete sequences in GenBank (Table 1). The RT-PCR conditions and reaction mixture were designed according to the SuperScript II One-Step RT-PCR System instructions (Invitrogen, Carlsbad, CA, USA). To identify the extreme 3′ genomic sequence, we used a modified RACE (3′ rapid amplification of cDNA ends) technique. The sense primer F5 (Table 1) was chosen from the ORF2 region, and the antisense primers included a commercially available anchored adaptor primer and an amplification primer (Invitrogen). Using inner PCR primers, we sequenced the PCR products of 5 fragments in both directions (Table 1); the sequence data were collected by an ABI3730 Genetic Analyzer (JinSiTe Biotech Co., Nanjing, China).
We assembled the near-complete genome of avian HEV, which was 6,660 nt long including the 3′ poly A tail, by using 5 overlapping fragments sequences and Lasergene 7.0 EditSeq computer programs (DNAStar, Madison, WI, USA) and designated it China avian HEV (CaHEV). CaHEV contained a complete ORF1 gene encoding a nonstructural protein of 1,522 aa, an ORF2 gene encoding a capsid protein of 606 aa, an ORF3 gene encoding a cytoskeleton-associated phosphoprotein of 87 aa, and a 3′ noncoding region of 121 nt. The sequences of CaHEV were deposited into GenBank under accession no. GU954430.
The near-complete genomic and different region sequence analyses performed by using ClustalW (www.clustal.org) and Lasergene 7.0 MegAlign software indicated that CaHEV shared the highest identity (98.3%) with European avian HEV isolate (EaHEV) and 82.0%–82.6% with 3 other avian HEV isolates (Table 2). Moreover, compared with the different regions of 4 other avian HEV strains, the ORF1 gene of CaHEV shared 80.7%–98.3% nt and 92.7%–98.8% aa sequence identities, the ORF2 gene shared 84.1%–98.5% nt and 98.3%–99.7% aa sequence identities, the ORF3 gene shared 93.9%–98.9% nt and 88.6%– 97.7% aa identities, and the 3′ noncoding region shared 78.9%–97.6% nt identities (Table 2).
ORF1 of CaHEV contained most mutations compared with prototype avian HEV (prototype aHEV); 5, 16, and 29 nonsilent mutations occurred in the methyltransferase, helicase, and RNA-dependent RNA polymerase (RdRp) functional domains, respectively (data not shown). However, only 2 mutations occurred in motif VII of RdRp domain (Figure 1, panel A), which contains 8 motifs responsible for virus replication (13). The 2 mutations in motif VII of the CaHEV RdRp domain are L(1432)M and I(1434)V. Australian avian HEV isolate (AaHEV) also has the mutation in the latter position and was a transition from I(1433) to T (Figure 1, panel A). This position is well conserved among mammalian HEV isolates by the presence of V, which is the same as CaHEV (Figure 1, panel A).
In the ORF2 region, 6 nonsilent mutations (C4R, R5G, G27S, T42A, T303V, and Q473M) were determined for CaHEV and compared with prototype aHEV. One mutation of Q(473)M, in the antigenic domain II, was seen in EaHEV and in CaHEV (Figure 1, panel B). Because this domain is unique to avian HEV, as predicted by Haqshenas et al. (14) and Guo et al. (10), this point mutation may change the antigenicity of the epitopes in domain II of the capsid protein. In antigenic domain IV, a mutation of R(600)K occurred in the “avirulent aHEV” compared with other 4 avian HEV strains, including CaHEV, from the sick chickens (Figure 1, panel C). This mutation may affect the virulence of avian HEV as speculated by Billam et al. (8) and Billic et al. (9). The 3 putative N-linked glycosylation sites (255NLS , 510NST , and 522NGS ) are shared between prototype aHEV and CaHEV (data not shown). However, the second site is 510NNT in “avirulent aHEV” and AaHEV strains and is eliminated in the EaHEV strain (9). In human and swine HEV strains, these sites are 137NLS (1), 310NLT (2), and 562NLS (3). Recently, the potential N-linked glycosylation in ORF2 was shown to prevent formation of infectious particles, but its role in other functions of HEV, e.g., virus virulence and cell tropism, remain to be elucidated (15). In the ORF3 gene, including only 83 aa, 10 nonsilent mutations were found compared with the prototype aHEV, and 9 mutations were the same as EaHEV (data not shown).
Phylogenetic trees of the near full-length sequence of avian and mammalian HEV strains were constructed by using the neighbor-joining distance method and Lasergene 7.0 software. A bootstrap test of 1,000 replicates was used to evaluate the reliability of the groups. Avian HEV was segregated into a distinct branch separate from mammalian HEV; according to the genotype separation corresponding to their geographic origin suggested by Bilic et al. (9), CaHEV belongs to avian HEV genotype 3 (Figure 2).
Avian HEV infection of a chicken flock in Shandong, China, was identified by detection of avian HEV ORF2 antibodies and viral RNA. A near-complete avian HEV genome from the flock was determined, and sequence analysis indicated that this avian HEV strain displayed the highest identity (98.3%) with EaHEV and belonged to avian HEV genotype 3.
Mr Qin Zhao is a PhD student in the laboratory of “Taishan Scholar” Immunobiology at the College of Veterinary Medicine, Shandong Agricultural University. His research interests are avian HEV pathogenesis and immunology.
This study was partially funded by the Taishan Scholar project of Shandong Province.
- Emerson SU, Anderson D, Arankalle A, Meng XJ, Purdy M, Schlauder GG, Genus Hepevirus. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editors. Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. London: Elsevier Academic Press; 2004. p. 853–7.
- Haqshenas G, Shivaprasad HL, Woolcock PR, Read DH, Meng XJ. Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States. J Gen Virol. 2001;82:2449–62.[REMOVED HYPERLINK FIELD]
- Payne CJ, Plant SL, Ellis TM, Hillier PW, Hopkinson W. The detection of the big liver and spleen agent in infected tissues via intravenous chick-embryo inoculation. Avian Pathol. 1993;22:245–56.
- Ritchie SJ, Riddell C. “Hepatitis–splenomegaly” syndrome in commercial egg laying hens. Can Vet J. 1991;32:500–1.
- Huang FF, Haqshenas G, Shivaprasad HL, Guenette PR, Woolcock PR, Larsen CT, Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States. J Clin Microbiol. 2002;40:4197–202.
- Peralta B, Biarnés M, Ordóñez G, Porta R, Martín M, Mateu E, Evidence of widespread infection of avian hepatitis E virus (avian HEV) in chickens from Spain. Vet Microbiol. 2009;137:31–6.
- Huang FF, Sun ZF, Emerson SU, Purcell RH, Shivaprasad HL, Pierson FW, Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. J Gen Virol. 2004;85:1609–18.
- Billam P, Sun ZF, Meng XJ. Analysis of the complete genomic sequence of an apparently avirulent strain of avian hepatitis E virus (avian HEV) identified major genetic differences compared with the prototype pathogenic strain of avian HEV. J Gen Virol. 2007;88:1538–44.
- Bilic I, Jaskulska B, Basic A, Morrow CJ, Hess M. Sequence analysis and comparison of avian hepatitis E viruses from Australia and Europe indicate the existence of different genotypes. J Gen Virol. 2009;90:863–73.
- Guo H, Zhou EM, Sun ZF, Meng XJ, Halbur PG. Identification of B-cell epitopes in the capsid protein of avian hepatitis E virus (avian HEV) that are common to human and swine HEVs or unique to avian HEV. J Gen Virol. 2006;87:217–23.
- Zhang L, Zhou EM, Cui ZZ, Dong SW, Jing ST, Peng J, Serological investigation of avian hepatitis E virus infection and its relationship with chicken hepatitis–splenomegaly syndrome [in Chinese]. China Poultry. 2008;30:22–4.
- Sun ZF, Larsen CT, Dunlop A, Huang FF, Pierson FW, Toth TE, Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis–splenomegaly syndrome in different geographical regions of the United States. J Gen Virol. 2004;85:693–700.
- Koonin EV, Gorbalenya AE, Purdy MA, Rozanov MN, Reyes GR, Bradley DW. Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses. Proc Natl Acad Sci U S A. 1992;89:8259–63.
- Haqshenas G, Huang FF, Fenaux M, Guenette DK, Pierson FW, Larsen CT, The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and chicken big liver and spleen disease virus. J Gen Virol. 2002;83:2201–9.
- Graff J, Zhou YH, Torian U, Nguyen H, St Claire M, Yu C, Mutations within potential glycosylation sites in the capsid protein of hepatitis E virus prevent the formation of infectious virus particles. J Virol. 2008;82:1185–94.
- Figure 1. Amino acid sequence comparison of motif VII in the open reading frame (ORF) 1 RNA-dependent RNA polymerase (RdRp) region of avian, human, and swine hepatitis E viruses (HEVs) (A),...
- Figure 2. Phylogenetic trees based on the near-complete genomic sequences of avian hepatitis E virus (HEV) and 10 human and swine HEV isolates. GenBank accession numbers follow the name of HEV...
- Table 1. Primers used for PCR amplification of the China avian hepatitis E virus genome
- Table 2. Percentage identities among avian HEV strains in nucleotide/amino acid sequences
Suggested citation for this article: Zhao Q, Zhou EM, Dong SW, Qiu HK, Zhang L, Hu SB, et al. Analysis of avian hepatitis E virus from chickens, China. Emerg Infect Dis [serial on the Internet]. 2010 Sep [date cited]. http://dx.doi.org/10.3201/eid1609.100626
1Current affiliation: Northwest A&F University, Yangling, People’s Republic of China.
Please use the form below to submit correspondence to the authors or contact them at the following address:
En Min Zhou, College of Veterinary Medicine, Northwest A&F University, 22 Xinong Rd, Yangling, Shaanxi Province, 712100, People’s Republic of China
Comment submitted successfully, thank you for your feedback.
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
- Page created: August 28, 2011
- Page last updated: August 28, 2011
- Page last reviewed: August 28, 2011
- Centers for Disease Control and Prevention,
National Center for Emerging and Zoonotic Infectious Diseases (NCEZID)
Office of the Director (OD)