Molecular Typing of Protease-Resistant Prion Protein in Transmissible Spongiform Encephalopathies of Small Ruminants, France, 2002–2009
Figure 4. Western blot analysis of protease-resistant prion protein in 2 CH1641-like sheep isolates (06-017, lane 3; 06-287, lane 4) detected by Bar233 (A), P4 (B), and SAF84 (C) antibodies. These samples were compared with 2 sheep-passaged scrapie isolates (SSBP/1, lane 1; CH1641, lane 5) and an isolate from a sheep experimentally infected with classical spongiform encephalopathy (SB1, lane 2). Samples in panel C were deglycosylated with peptide N-glycosidase F before Western blot analysis.