Emerging Infectious Disease ISSN: 1080-6059

Molecular Typing of Protease-Resistant Prion Protein in Transmissible Spongiform Encephalopathies of Small Ruminants, France, 2002–2009

Johann Vulin, Anne-Gaëlle Biacabe, Géraldine Cazeau, Didier Calavas, and Thierry Baron

Author affiliations: Author affiliation: Agence Nationale de Sécurité Sanitaire, Lyon, France

Figure 4

Western blot analysis of protease-resistant prion protein in 2 CH1641-like sheep isolates (06-017, lane 3; 06-287, lane 4) detected by Bar233 (A), P4 (B), and SAF84 (C) antibodies. These samples were compared with 2 sheep-passaged scrapie isolates (SSBP/1, lane 1; CH1641, lane 5) and an isolate from a sheep experimentally infected with classical spongiform encephalopathy (SB1, lane 2). Samples in panel C were deglycosylated with peptide N-glycosidase F before Western blot analysis.

Figure 4. Western blot analysis of protease-resistant prion protein in 2 CH1641-like sheep isolates (06-017, lane 3; 06-287, lane 4) detected by Bar233 (A), P4 (B), and SAF84 (C) antibodies. These samples were compared with 2 sheep-passaged scrapie isolates (SSBP/1, lane 1; CH1641, lane 5) and an isolate from a sheep experimentally infected with classical spongiform encephalopathy (SB1, lane 2). Samples in panel C were deglycosylated with peptide N-glycosidase F before Western blot analysis.

Main Article

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.