Figure 5. Western blot analysis of protease-resistant prion protein in 2 goat isolates (CH636, lane 3; 08-357, lane 4) detected by Bar233 (A), P4 (B), and SAF84 (C) antibodies. These samples were compared with an isolate from a goat naturally infected with scrapie (lane 1); an isolate from a goat experimentally infected with classical BSE (CH41x76, lane 2); and a sheep-passaged scrapie isolate (CH1641, lane 5). Samples in panel C were deglycosylated with peptide N-glycosidase F before Western blot analysis.
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