Isolation and Phylogenetic Grouping of Equine Encephalosis Virus in Israel
Karin Aharonson-Raz, Amir Steinman, Velizar Bumbarov, Sushila Maan, Narender Singh Maan, Kyriaki Nomikou, Carrie Batten, Christiaan Potgieter, Yuval Gottlieb, Peter Mertens, and Eyal Klement
Author affiliations: The Hebrew University, Rehovot, Israel (K. Aharonson-Raz, A. Steinman, Y. Gottlieb, E. Klement); Kimron Veterinary Institute, Bet Dagan, Israel (V. Bumbarov); Institute for Animal Health, Pirbright, UK (S. Maan, N.S. Maan, K. Nomikou, C. Batten, P. Mertens); Deltamune (Pty) Ltd, Lyttelton, South Africa (C. Potgieter)
Figure 2. Phylogeny of equine encephalosis virus (EEV) segment 10 (nonstructural protein 3 gene) isolated from horses in Israel in 2009. The phylogenic tree was constructed by using the neighbor-joining method and bootstrapped with 100 replicates. Branch lengths are indicative of the genetic distances between sequences. Other orbiviruses were included for reference, with Broadhaven virus (BRDV) selected as the outgroup. The 3 suggested EEV clusters are marked green (cluster A), blue (cluster B), and orange (cluster C, representing the isolates from Israel). REF, reference; FLD, field strain; H3, H5, H8, horse 3, 5, and 8; EHDV, epizootic hemorrhagic disease virus; BTV, bluetongue virus; PALV, Palyam virus; AHSV, African horse sickness virus. Scale bar indicates nucleotide substitution per site.
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