New Dengue Virus Type 1 Genotype in Colombo, Sri Lanka
Hasitha A. Tissera, Eng Eong Ooi, Duane J. Gubler, Ying Tan, Barathy Logendra, Wahala M.P.B. Wahala, Aravinda M. de Silva, M.R. Nihal Abeysinghe, Paba Palihawadana, Sunethra Gunasena, Clarence C. Tam, Ananda Amarasinghe, G. William Letson, Harold S. Margolis, and Aruna Dharshan De Silva
Author affiliations: Ministry of Health, Colombo, Sri Lanka (H.A. Tissera, M.R.N. Abeysinghe, P. Palihawadana); London School of Hygiene and Tropical Medicine, London, UK (H.A. Tissera, C.C. Tam); Duke–National University of Singapore Graduate Medical School, Singapore (E.E. Ooi, D.J. Gubler, Y. Tan); Genetech Research Institute, Colombo (B. Logendra, A.D. De Silva); University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA (W.M.P.B. Wahala, A.M. de Silva); Medical Research Institute, Colombo (S. Gunasena); International Vaccine Institute, Seoul, South Korea (A. Amerasinghe, G.W. Letson, H.S. Margolis)
Figure. Phylogenetic tree of dengue virus 1 (DENV-1) serotype viruses from Sri Lanka (SL), 2009–2010, and other DENV-1 viruses. The tree is based on a 498-bp (nt 2056–2554) fragment that encodes portions of the envelope protein and nonstructural protein 1. Phylogenetic analysis was conducted by using MEGA5 (10). Percentages of replicate trees in which the associated taxa clustered in the bootstrap test (1,000 replicates) are shown next to the branches. Genotype I (Asia) includes SL isolates from 2009–2010; genotype III (South Pacific) includes SL isolates from the early 1980s; genotype IV (Africa/Americas) includes SL isolates from the 1990s and early 2000s. Classification and naming of DENV-1 genotypes are based on the report by Rico-Hesse (11). DV, dengue virus. Scale bar indicates nucleotide substitutions per site.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.