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Volume 17, Number 11—November 2011
CME ACTIVITY - Research

Close Similarity between Sequences of Hepatitis E Virus Recovered from Humans and Swine, France, 2008−2009

Jérôme Bouquet, Sophie Tessé, Aurélie Lunazzi, Marc Eloit, Nicolas Rose, Elisabeth Nicand, and Nicole PavioComments to Author 
Author affiliations: Anses, Laboratoire de Santé Animale, Maisons-Alfort, France (J. Bouquet, A. Lunazzi, N. Pavio); Hôpital des Armées Val de Grâce, Paris, France (S. Tessé, E. Nicand); Ecole Nationale Vétérinaire d’Alfort, Maisons-Alfort (M. Eloit); Anses, Laboratoire de Ploufragan-Plouzané, Ploufragan, France (N. Rose)

Main Article

Figure 3

Phylogenetic tree of hepatitis E virus (HEV) detected in human and swine constructed by the neighbor-joining method with a bootstrap of 1,000 replicates based on the ClustalW alignment (MEGA4, www.megasoftware.net) of 204- to 306-nt sequences within open reading frame 2. The 106 HEV sequences recovered from patients from France are displayed as black dots (GenBank accession nos. JF730329−JF730434), the 43 HEV sequences recovered from swine from France are displayed as red dots (accession nos. JF

Figure 3. Phylogenetic tree of hepatitis E virus (HEV) detected in human and swine constructed by the neighbor-joining method with a bootstrap of 1,000 replicates based on the ClustalW alignment (MEGA4, www.megasoftware.net) of 204- to 306-nt sequences within open reading frame 2. The 106 HEV sequences recovered from patients from France are displayed as black dots (GenBank accession nos. JF730329−JF730434), the 43 HEV sequences recovered from swine from France are displayed as red dots (accession nos. JF718787−JF718829), and the 22 reference strains from GenBank are displayed as white dots (GenBank accession nos. in Table A1). Genotype 4, subtypes 3f, 3c, and 3e, as defined by Lu et al. (8), are encircled by a solid black line; undefined subtype is encircled by a dashed black line. Bootstrap values >70% are indicated on respective branches. Scale bar represents nucleotide substitutions per site.

Main Article

References
  1. Panda  SK, Thakral  D, Rehman  S. Hepatitis E virus. Rev Med Virol. 2007;17:15180. DOIPubMedGoogle Scholar
  2. Purcell  RH, Emerson  SU. Hepatitis E: an emerging awareness of an old disease. J Hepatol. 2008;48:494503. DOIPubMedGoogle Scholar
  3. Dalton  HR, Bendall  R, Ijaz  S, Banks  M. Hepatitis E: an emerging infection in developed countries. Lancet Infect Dis. 2008;8:698709. DOIPubMedGoogle Scholar
  4. Emerson  SU, Anderson  D, Arankalle  A, Meng  XJ, Purdy  M, Schlauder  GG, Virus taxonomy VIIIth report of the ICTV. Hepevirus; 2004; 851–3.
  5. Lu  L, Li  C, Hagedorn  CH. Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol. 2006;16:536. DOIPubMedGoogle Scholar
  6. Pavio  N, Meng  X, Renou  C. Zoonotic hepatitis E: animal reservoirs and emerging risks. Vet Res. 2010;41:46. DOIPubMedGoogle Scholar
  7. Li  TC, Chijiwa  K, Sera  N, Ishibashi  T, Etoh  Y, Shinohara  Y, Hepatitis E virus transmission from wild boar meat. Emerg Infect Dis. 2005;11:195860.PubMedGoogle Scholar
  8. Tei  S, Kitajima  N, Takahashi  K, Mishiro  S. Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet. 2003;362:3713. DOIPubMedGoogle Scholar
  9. Meng  XJ, Purcell  RH, Halbur  PG, Lehman  JR, Webb  DM, Tsareva  TS, A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci U S A. 1997;94:98605. DOIPubMedGoogle Scholar
  10. Nakamura  M, Takahashi  K, Taira  K, Taira  M, Ohno  A, Sakugawa  H, Hepatitis E virus infection in wild mongooses of Okinawa, Japan: demonstration of anti-HEV antibodies and a full-genome nucleotide sequence. Hepatol Res. 2006;34:13740. DOIPubMedGoogle Scholar
  11. Munné  MS, Vladimirsky  S, Otegui  L, Castro  R, Brajterman  L, Soto  S, Identification of the first strain of swine hepatitis E virus in South America and prevalence of anti-HEV antibodies in swine in Argentina. J Med Virol. 2006;78:157983. DOIPubMedGoogle Scholar
  12. dos Santos  DRL, Vitral  CL, de Paula  VS, Marchevsky  RS, Lopes  JF, Gaspar  AMC, Serological and molecular evidence of hepatitis E virus in swine in Brazil. Vet J. 2009;182:47480. DOIPubMedGoogle Scholar
  13. Leblanc  D, Ward  P, Gagné  M, Poitras  E, Müller  P, Trottier  Y, Presence of hepatitis E virus in a naturally infected swine herd from nursery to slaughter. Int J Food Microbiol. 2007;117:1606. DOIPubMedGoogle Scholar
  14. McCreary  C, Martelli  F, Grierson  S, Ostanello  F, Nevel  A, Banks  M. Excretion of hepatitis E virus by pigs of different ages and its presence in slurry stores in the United Kingdom. Vet Rec. 2008;163:2615. DOIPubMedGoogle Scholar
  15. Banks  M, Bendall  R, Grierson  S, Heath  G, Mitchell  J, Dalton  H. Human and porcine hepatitis E virus strains, United Kingdom. Emerg Infect Dis. 2004;10:9535.PubMedGoogle Scholar
  16. van der Poel  WH, Verschoor  F, van der Heide  R, Herrera  MI, Vivo  A, Kooreman  M, Hepatitis E virus sequences in swine related to sequences in humans, the Netherlands. Emerg Infect Dis. 2001;7:9706. DOIPubMedGoogle Scholar
  17. Meng  XJ, Halbur  PG, Shapiro  MS, Govindarajan  S, Bruna  JD, Mushahwar  IK, Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J Virol. 1998;72:971421.PubMedGoogle Scholar
  18. Rutjes  SA, Lodder  WJ, Lodder-Verschoor  F, van den Berg  HHJL, Vennema  H, Duizer  E, Sources of hepatitis E virus genotype 3 in the Netherlands. Emerg Infect Dis. 2009;15:3817. DOIPubMedGoogle Scholar
  19. Widén  F, Sundqvist  L, Matyi-Toth  A, Metreveli  G, Belák  S, Hallgren  G, Molecular epidemiology of hepatitis E virus in humans, pigs and wild boars in Sweden. Epidemiol Infect. 2011;139:36171. DOIPubMedGoogle Scholar
  20. Reuter  G, Fodor  D, Forgách  P, Kátai  A, Szucs  G. Characterization and zoonotic potential of endemic hepatitis E virus (HEV) strains in humans and animals in Hungary. J Clin Virol. 2009;44:27781. DOIPubMedGoogle Scholar
  21. Zhang  W, Yang  S, Ren  L, Shen  Q, Cui  L, Fan  K, Hepatitis E virus infection in central China reveals no evidence of cross-species transmission between human and swine in this area. PLoS ONE. 2009;4:e8156. DOIPubMedGoogle Scholar
  22. Boutrouille  A, Bakkali-Kassimi  L, Crucière  C, Pavio  N. Prevalence of anti-hepatitis E virus antibodies in French blood donors. J Clin Microbiol. 2007;45:200910. DOIPubMedGoogle Scholar
  23. Mansuy  JM, Legrand-Abravanel  F, Calot  JP, Peron  JM, Alric  L, Agudo  S, High prevalence of anti-hepatitis E virus antibodies in blood donors from South West France. J Med Virol. 2008;80:28993. DOIPubMedGoogle Scholar
  24. Rose  N, Lunazzi  A, Dorenlor  V, Merbah  T, Eono  F, Eloit  M, High prevalence of hepatitis E virus in French domestic pigs. Comp Immunol Microbiol Infect Dis. 2001;34:41927. DOIPubMedGoogle Scholar
  25. Colson  P, Borentain  P, Queyriaux  B, Kaba  M, Moal  V, Gallian  P, Pig liver sausage as a source of hepatitis E virus transmission to humans. J Infect Dis. 2010;202:82534. DOIPubMedGoogle Scholar
  26. Ministére de l’Agriculture et de la Péche. Consommation de viande en France. AGRESTE, 2008. 2008/n°29 [cited 2008 Jun]. http://www.agreste.agriculture.gouv.fr/IMG/pdf/syntheseviande0806.pdf
  27. Cooper  K, Huang  FF, Batista  L, Rayo  CD, Bezanilla  JC, Toth  TE, Identification of genotype 3 hepatitis E virus (HEV) in serum and fecal samples from pigs in Thailand and Mexico, where genotype 1 and 2 HEV strains are prevalent in the respective human populations. J Clin Microbiol. 2005;43:16848. DOIPubMedGoogle Scholar
  28. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:15969. DOIPubMedGoogle Scholar
  29. French Ministry of the Economy. Finance and Industry. Decree no. 2009-1707 of 30 December 2009. Paris: Official Journal of the French Republic, Direction de l’information légale et administrative; 2010.
  30. Legrand-Abravanel  F, Mansuy  J, Dubois  M, Kamar  N, Peron  J, Rostaing  L, Hepatitis E virus genotype 3 diversity, France. Emerg Infect Dis. 2009;15:1104. DOIPubMedGoogle Scholar
  31. Renou  C, Moreau  X, Pariente  A, Cadranel  J, Maringe  E, Morin  T, A national survey of acute hepatitis E in France. Aliment Pharmacol Ther. 2008;27:108693. DOIPubMedGoogle Scholar
  32. Peralta  B, Mateu  E, Casas  M, de Deus  N, Martín  M, Pina  S. Genetic characterization of the complete coding regions of genotype 3 hepatitis E virus isolated from Spanish swine herds. Virus Res. 2009;139:1116. DOIPubMedGoogle Scholar
  33. Adlhoch  C, Wolf  A, Meisel  H, Kaiser  M, Ellerbrok  H, Pauli  G. High HEV presence in four different wild boar populations in East and West Germany. Vet Microbiol. 2009;139:2708. DOIPubMedGoogle Scholar
  34. Di Bartolo  I, Ponterio  E, Castellini  L, Ostanello  F, Ruggeri  FM. Viral and antibody HEV prevalence in swine at slaughterhouse in Italy. Vet Microbiol. 2011;149:3308. DOIPubMedGoogle Scholar
  35. Forgách  P, Nowotny  N, Erdélyi  K, Boncz  A, Zentai  J, Szucs  G, Detection of hepatitis E virus in samples of animal origin collected in Hungary. Vet Microbiol. 2010;143:10616. DOIPubMedGoogle Scholar
  36. Grandadam  M, Tebbal  S, Caron  M, Siriwardana  M, Larouze  B, Koeck  JL, Evidence for hepatitis E virus quasispecies. J Gen Virol. 2004;85:318994. DOIPubMedGoogle Scholar
  37. de Deus  N, Casas  M, Peralta  B, Nofrarías  M, Pina  S, Martín  M, Hepatitis E virus infection dynamics and organic distribution in naturally infected pigs in a farrow-to-finish farm. Vet Microbiol. 2008;132:1928. DOIPubMedGoogle Scholar
  38. Bouwknegt  M, Rutjes  SA, Reusken  CB, Stockhofe-Zurwieden  N, Frankena  K, de Jong  MCM, The course of hepatitis E virus infection in pigs after contact-infection and intravenous inoculation. BMC Vet Res. 2009;5:7. DOIPubMedGoogle Scholar

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Page updated: October 27, 2011
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