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Volume 17, Number 11—November 2011
THEME ISSUE
CHOLERA IN HAITI

Research

Characterization of Toxigenic Vibrio cholerae from Haiti, 2010–2011

Deborah TalkingtonComments to Author , Cheryl Bopp, Cheryl Tarr, Michele B. Parsons, Georges Dahourou, Molly Freeman, Kevin Joyce, Maryann Turnsek, Nancy Garrett, Michael Humphrys, Gerardo Gomez, Steven Stroika, Jacques Boncy, Benjamin Ochieng, Joseph Oundo, John Klena, Anthony Smith, Karen Keddy, and Peter Gerner-Smidt
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (D. Talkington, C. Bopp, C. Tarr, M.B. Parsons, M. Freeman, K. Joyce, M. Turnsek, N. Garrett, M. Humphrys, G. Gomez, S. Stroika, P. Gerner-Smidt); Centers for Disease Control and Prevention, Port-au-Prince, Haiti (G. Dahourou); CDC–Kenya Medical Research Institute, Kisumu, Kenya (B. Ochieng, J. Oundo); Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China (J. Klena); Ministry of Public Health and Population, Port-au-Prince (J. Boncy); National Institute for Communicable Diseases, Johannesburg, South Africa (A. Smith, K. Keddy)

Main Article

Table 5

Sequencing and PCR results for Vibrio cholerae isolates collected during 1991–2011*

Location of origin Sequencing results
PCR results
No. sequenced for ctxB ctxB
allele No. sequenced for tcpA tcpA
allele No. tested for rstR rstR allele No. tested for VC2346 gene VC2346 gene
Haiti 56 B-7 56 ETCIRS 56 ET 56 Pos
Afghanistan 2 B-1 2 ET, ETCIRS 2 ET 2 Pos
Cameroon† 1 B-7 1 ETCIRS 1 ET 1 Pos
Djibouti 2 B-1 2 ET 2 ET 2 Pos
Ethiopia 1 B-1 1 ET 1 ET 1 Pos
India† 6 B-1 (3), B-7 (3) 6 ETCIRS 6 ET 6 Pos
Kenya 14 B-1 14 ET 2 ET 14 Pos
Nepal† 1 B-7 1 ETCIRS 1 ET 1 Pos
Nigeria 2 B-1 2 ET, ETCIRS 2 ET 2 Pos
Oman 1 B-1 1 ET 1 ET 1 Pos
Pakistan 6 B-1 6 ETCIRS 6 ET 6 Pos
Peru 2 B-3 2 ET 2 ET 2 Pos
Somalia 2 B-1 2 ET 2 ET 2 Pos
South Africa 2 B-1 2 ETCIRS 2 ET 2 Pos
Sri Lanka 1 B-1 1 ETCIRS 1 ET 1 Pos
Sudan 5 B-1 5 ET 2 ET 2 Pos
Togo 2 B-1 2 ET 2 ET 2 Pos
US Gulf Coast
1
B-1
1
ET

1
Classic
1
Neg
Total 107 107 92 104

*ctxB, cholera toxin subunit B; tcpA, biotyping gene; rstR, biotype-specific repeat sequence transcriptional regulator; Pos, positive; Neg, negative.
†By pulsed-field gel electrophoresis and ctxB and tcpA sequencing, the isolates from Cameroon and Nepal and 3 isolates from India are most closely related to the isolates from Haiti.

*ctxB, cholera toxin subunit B; tcpA, biotyping gene; rstR, biotype-specific repeat sequence transcriptional regulator; Pos, positive; Neg, negative.
†By pulsed-field gel electrophoresis and ctxB and tcpA sequencing, the isolates from Cameroon and Nepal and 3 isolates from India are most closely related to the isolates from Haiti.

*ctxB, cholera toxin subunit B; tcpA, biotyping gene; rstR, biotype-specific repeat sequence transcriptional regulator; Pos, positive; Neg, negative.
†By pulsed-field gel electrophoresis and ctxB and tcpA sequencing, the isolates from Cameroon and Nepal and 3 isolates from India are most closely related to the isolates from Haiti.

*ctxB, cholera toxin subunit B; tcpA, biotyping gene; rstR, biotype-specific repeat sequence transcriptional regulator; Pos, positive; Neg, negative.
†By pulsed-field gel electrophoresis and ctxB and tcpA sequencing, the isolates from Cameroon and Nepal and 3 isolates from India are most closely related to the isolates from Haiti.

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