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Volume 17, Number 12—December 2011

Research

Isolation of Prion with BSE Properties from Farmed Goat

John SpiropoulosComments to Author , Richard Lockey, Rosemary E. Sallis, Linda A. Terry, Leigh Thorne, Thomas M. Holder, Katy E. Beck, and Marion M. Simmons
Author affiliations: Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey, UK

Main Article

Table

Bioassay results of first passage (ruminant to mouse) to determine presence of BSE*

Sample source and treatment† Tissue‡ Mouse line No. PrPSc-positive mice/total no. mice inoculated§ Clinically positive and H&E-positive mice Mean (SD) incubation period postinoculation, d
Caprine BSE suspected (V459/90) (16)
Fixed and embedded Brainstem and cerebellum C57/BL6 5/20 0 NA
RIII 5/20 3 525 (93)
VM 9/20 6 620 (32)
tg338 8/10 0 NA
Natural caprine scrapie 1 (E90/89) (16)
Fixed and embedded Brainstem C57/BL6 0/20 0 NA
RIII 0/20 0 NA
VM 0/20 0 NA
tg338 6/10 0 NA
Natural caprine scrapie 2 (84/1549) (17)
Fixed and embedded Brainstem C57/BL6 18/20 18 506 (26)
RIII 8/20 2 413 (98)
VM 15/20 6 586 (87)
tg338 10/10 5 196 (13)
Experimental caprine BSE (45x48) (21)
Fixed and embedded Brainstem C57/BL6 2/20 1 588 (NA)
RIII 3/20 2 535 (5)
VM 6/20 4 592 (51)
tg338 10/10 0 NA
Experimental ovine BSE (PG0341/00) (11)
Fixed and embedded Medulla RIII 7/20 6 388 (23)
tg338 5/5 0 NA
Frozen Medulla RIII 6/20 0 NA
tg338 NA NA >785¶
Natural bovine BSE (PG0475/05, UK passive surveillance)
Fixed and embedded Medulla C57/BL6 2/20 1 713 (NA)
RIII 6/20 5 477 (49)
VM 13/20 2 578 (35)
tg338 7/10 0 NA
Frozen Medulla C57/BL6 7/20 3 535 (76)
RIII 10/20 9 431 (29)
VM 16/20 3 568 (31)
tg338 6/10 0 NA
Natural ovine scrapie (PG2413/98, UK passive surveillance)
Fixed and embedded Medulla RIII 0/20 0 NA
tg338 5/5 3 97 (2)
Frozen Medulla RIII 9/20 8 490 (31)
tg338 10/10 10 64 (2)

*BSE, bovine spongiform encephalopathy; PrP, host-encoded prion protein; H&E, hematoxylin and eosin; NA, not applicable; UK, United Kingdom.
†Animal identification numbers are indicated in parentheses and, where available, references are supplied.
‡Brainstem inocula were prepared by pooling separate samples of brainstem (including medulla, midbrain, and thalamus) retrieved from paraffin wax blocks.
§Positive for PrPSc by using immunohistochemical analysis.
¶Postinoculation time, after which 2 of 10 inoculated mice were still alive at the time of writing; 5 of the 8 mice that died were PrPSc positive when examined by using immunohistochemical analysis.

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