Candidate Cell Substrates, Vaccine Production, and Transmissible Spongiform Encephalopathies
Figure 4. Western blot of brain extract from C57/Bl mouse inoculated with 22L mouse-adapted scrapie agent (lanes 1, 2); NIH-3T3 cells exposed to normal mouse brain and passaged 30 times (lane 3); NIH-3T3 (lane 4) and L929 (lane 5) cells exposed to 22L scrapie agent and passaged 30 times. Nonproteinase K [PK]–treated samples (lane 1), PK-treated samples (lanes 2–5). Western blots were probed with prion protein monoclonal antibody 6H4.