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Volume 17, Number 12—December 2011

Research

Lineage and Virulence of Streptococcus suis Serotype 2 Isolates from North America

Nahuel Fittipaldi, Jiangu Xu, Sonia Lacouture, Prasit Tharavichitkul, Makoto Osaki, Tsutomu Sekizaki, Daisuke Takamatsu, and Marcelo GottschalkComments to Author 
Author affiliations: Université de Montréal, St-Hyacinthe, Quebec, Canada (N. Fittipaldi, S. Lacouture, M. Gottschalk); National Institute for Communicable Disease Control and Prevention, Beijing, People’s Republic of China (J. Xu); Chiang Mai University, Chiang Mai, Thailand (P. Tharavichitkul); Agriculture, Forestry and Fisheries Research Council, Tokyo, Japan (M. Osaki); The University of Tokyo, Tokyo (T. Sekizaki); National Institute of Animal Health, Tsukuba, Japan (D. Takamatsu)

Main Article

Table 3

Association of Streptococcus suis serotype 2 STs and srtF and srtG pilus clusters in isolates from North America*

ST No. strains srtF pilus cluster
srtG pilus cluster
Gene†
Pili expression‡, Sfp1 Gene†
Pili expression, Sgp1‡
srtF sfp1 sfp2 sipF srtG sgp1 sgp2
1 11 11 11 11 11 11 3 3 3 0
25 36 36 36 36 36 0 36 36 36 36
28 49 49 49 49 49 46 49 49 49 47

*ST, sequence type.
†The presence of the genes was detected by PCR by using primers and conditions described by Takamatsu et al. (13).
‡Expression of pili encoded by the srtF and srtG pilus clusters was performed by Western blotting by using described antibodies directed against the major subunits of these structures (16,17).

Main Article

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