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Volume 17, Number 12—December 2011

Letter

Novel Astroviruses in Children, Egypt

Salwa F. AhmedComments to Author , Peter J. Sebeny, John D. Klena, Guillermo Pimentel, Adel Mansour, Amel M. Naguib, Jody Bruton, Sylvia Y.N. Young, Lori R. Holtz, and David Wang
Author affiliations: US Naval Medical Research Unit No.3, Cairo, Egypt (S.F. Ahmed, P.J. Sebeny, J.D. Klena, G. Pimentel, A. Mansour, J. Bruton, S.Y.N. Young); Ministry of Health, Cairo (A.M. Naguib); Washington University School of Medicine, St. Louis, Missouri, USA (L.R. Holtz, D. Wang)

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Figure

A) Phylogenetic analysis of the partial amino acid sequences of the open reading frame (ORF) 2 capsid region of human astrovirus (HAstV) types I–VIII (using Mon primer) with other sequences from GenBank. B, C) Phylogenetic trees based on partial nucleotide sequences of MLB1 ORF2 (B) and ORF1b (VA2) (C). Egyptian isolates are shown in boldface. Sequence alignment was performed by using ClustalW in the BioEdit software package (www.clustal.org). Dendrograms were constructed by using the neighbor-j

Figure. A) Phylogenetic analysis of the partial amino acid sequences of the open reading frame (ORF) 2 capsid region of human astrovirus (HAstV) types I–VIII (using Mon primer) with other sequences from GenBank. B, C) Phylogenetic trees based on partial nucleotide sequences of MLB1 ORF2 (B) and ORF1b (VA2) (C). Egyptian isolates are shown in boldface. Sequence alignment was performed by using ClustalW in the BioEdit software package (www.clustal.org). Dendrograms were constructed by using the neighbor-joining method as in MEGA4 (www.megasoftware.net). Values on the branches represent the bootstrap values from 2,000 replicates. Scale bar in A indicates amino acid substitutions per site; scale bars in B and C indicate nucleotide substitutions per site.

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