Volume 17, Number 2—February 2011
Research
Phocine Distemper Virus in Seals, East Coast, United States, 2006
J.A. Philip Earle, Mary M. Melia, Nadine V. Doherty, Ole Nielsen, and S. Louise Cosby
Table
Phocine distemper virus primers for reverse transcription–PCR and DNA sequencing*
| Gene | Primer sequence, 5′ → 3′ | Gene | Primer sequence, 5′ → 3′ |
|---|---|---|---|
| P forward | ATGTTTATGATCACAGCGGT | F4 reverse | CCCGTAAACTTGGTCCAA |
| P reverse | ATTGGGTTGCACCACTTGTC | F5 forward | ATAATATAGGGTCACAGG |
| M forward | ATACTGCATTAACCCTGG | F5 reverse | CCTGTGACCCTATATTAT |
| M reverse | TTAGGTTGTTGGTCTTGGTAG | F6 forward | TTGTGCTTCTATCTTGTG |
| M1 forward | ATAACGATGATCTTGGCC | F6 reverse | CACAAGATAGAAGCACAA |
| M1 reverse | GGCCAAGATCATCGTTAT | F7 forward | GAATCCTCTGATCAAATC |
| M2 forward | ACACAGCTCAGAGATTCC | F7 reverse | GATTTGATCAGAGGATTC |
| M2 reverse | GGAATCTCTGAGCTGTGT | F8 forward | TGCAAGCTGGCACATCAG |
| M3 forward | ACTGGTGTTCGCCCTTGG | F8 reverse | CTGATGTGCCAGCTTGCA |
| M3 reverse | CCAAGGGCGAACACCAGT | H forward | CGAGGTTGAGGAAAGAAG |
| M4 forward | TTAAATTCCCAGTTCTTG | H reverse | CTCAATCTCGGTGGGTAC |
| M4 reverse | CAAGAACTGGGAATTTAA | H1 forward | AGGCAGTGCATCATCAAG |
| M5 forward | AGCCACTTGAATCTACGG | H1 reverse | CTTGATGATGCACTGCCT |
| M5 reverse | CCGTAGATTCAAGTGGCT | H2 forward | CAATCCTCTTGCTGACAC |
| F forward | GAGATTTGTGCACCTTTC | H2 reverse | GTGTCAGCAAGAGGATTG |
| F reverse | GC ATTGTTCTTGTAAAAGGC | H3 forward | AGATGGCTAGGTGATATG |
| F1 forward | TCATAGTCTCGATTCACC | H3 reverse | CATATCACCTAGCCATCT |
| F1 reverse | GGTGAATCGAGACTATGA | H4 forward | CACCGGGGTTTCATAAAG |
| F2 forward | TTATCAACAATTGGAATC | H4 reverse | CTTTATGAAACCCCGGTG |
| F2 reverse | GATTCCAATTGTTGATAA | H5 forward | GGATTATTATGAGGGTAC |
| F3 forward | TGCAGGTGCAGCTCTAGG | H5 reverse | CAATAGCATGATCACTCC |
| F3 reverse | CCTAGAGCTGCACCTGCA | H6 forward | GGAGTGATCATGCTATTG |
| F4 forward | TTGGACCAAGTTTACGGG | H6 reverse | GTACCCTCATAATAATCC |
*H, hemagglutinin; P, phosphoprotein; F, fusion; M, matrix.
1These authors contributed equally to this article.
