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Volume 17, Number 2—February 2011

Dispatch

Unusual Transmission of Plasmodium falciparum, Bordeaux, France, 2009

Marc-Olivier Vareil, Olivier Tandonnet, Audrey Chemoul, Hervé Bogreau, Mélanie Saint-Léger, Maguy Micheau, Pascal Millet, Jean-Louis Koeck, Alexandre Boyer, Christophe Rogier, and Denis MalvyComments to Author 
Author affiliations: Author affiliations: Centre Hospitalier Universitaire Pellegrin, Bordeaux, France (M.-O. Vareil, M. Saint-Léger, A. Boyer); Hôpital d’Enfants, Bordeaux (O. Tandonnet, M. Micheau); Hôpital d’Instruction des Armées, Bordeaux (A. Chemoul, J.-L. Koeck); Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France (H. Bogreau, C. Rogier); Université Victor Segalen, Bordeaux (P. Millet, D. Malvy); Centre Hospitalier St-André, Bordeaux (D. Malvy)

Main Article

Table

Genotyping results for 2 Plasmodium falciparum isolates, France, 2009*

Locus
Secondary case
Index case
Type
Allele
Type
Allele
Microsatellites†
7A11 NA 119 bp NA 119 bp
C4M79 NA 203 bp NA 203 bp
Pf2802 NA 139 bp NA 139 bp
Pf2689
NA
87 bp

NA
87 bp
Antigen genes‡
msp-1 Ro33 149 bp Ro33 149 bp
msp-2
FC27
413 bp

FC27
413 bp
Drug resistance genes§
pfcrt codon 76 Mutated ACA Mutated ACA
pfdhfr
Codon 16 Wild-type GCA Wild-type GCA
Codon 51 Mutated ATT Mutated ATT
Codon 59 Mutated CGT Mutated CGT
Codon 108 Mutated AAC Mutated AAC
Codon 164 Wild-type ATA Wild-type ATA

*NA, not applicable; msp, merozoite surface protein; pfcrt, P. falciparum chloroquine resistance transporter; pfdhfr, P. falciparum dihydrofolate reductase.
†Size of amplified DNA fragment expressed.
‡Allelic family and size of amplified DNA fragment expressed.
§Single-nucleotide polymorphism. pfcrt codon 76 point mutation was genotyped by nested PCR and restriction fragment-length polymorphism analysis. pfdhfr point mutations at positions 16, 51, 59, 108, and 164 were genotyped by nested PCR and primer expansion. Underlining indicates nucleotide positions that can be mutated.

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