Volume 17, Number 2—February 2011
Dispatch
Unusual Transmission of Plasmodium falciparum, Bordeaux, France, 2009
Marc-Olivier Vareil, Olivier Tandonnet, Audrey Chemoul, Hervé Bogreau, Mélanie Saint-Léger, Maguy Micheau, Pascal Millet, Jean-Louis Koeck, Alexandre Boyer, Christophe Rogier, and Denis Malvy
Table
Genotyping results for 2 Plasmodium falciparum isolates, France, 2009*
| Locus |
Secondary case | Index case | |||
|---|---|---|---|---|---|
| Type |
Allele |
Type |
Allele |
||
| Microsatellites† | |||||
| 7A11 | NA | 119 bp | NA | 119 bp | |
| C4M79 | NA | 203 bp | NA | 203 bp | |
| Pf2802 | NA | 139 bp | NA | 139 bp | |
| Pf2689 |
NA |
87 bp |
NA |
87 bp |
|
| Antigen genes‡ | |||||
| msp-1 | Ro33 | 149 bp | Ro33 | 149 bp | |
| msp-2 |
FC27 |
413 bp |
FC27 |
413 bp |
|
| Drug resistance genes§ | |||||
| pfcrt codon 76 | Mutated | ACA | Mutated | ACA | |
| pfdhfr | |||||
| Codon 16 | Wild-type | GCA | Wild-type | GCA | |
| Codon 51 | Mutated | ATT | Mutated | ATT | |
| Codon 59 | Mutated | CGT | Mutated | CGT | |
| Codon 108 | Mutated | AAC | Mutated | AAC | |
| Codon 164 | Wild-type | ATA | Wild-type | ATA | |
*NA, not applicable; msp, merozoite surface protein; pfcrt, P. falciparum chloroquine resistance transporter; pfdhfr, P. falciparum dihydrofolate reductase.
†Size of amplified DNA fragment expressed.
‡Allelic family and size of amplified DNA fragment expressed.
§Single-nucleotide polymorphism. pfcrt codon 76 point mutation was genotyped by nested PCR and restriction fragment-length polymorphism analysis. pfdhfr point mutations at positions 16, 51, 59, 108, and 164 were genotyped by nested PCR and primer expansion. Underlining indicates nucleotide positions that can be mutated.
