Blastomycosis in Man after Kinkajou Bite
Figure 2. Histopathologic and electron microscopic appearance of Blastomyces dermatitidis in kinkajou (Potos flavus) tissues. A) Lung showing B. dermatitidis yeast forms filling alveolar spaces. Alveolar septa are indicated by arrows. B) Lung showing yeast forms of B. dermatitidis. Inset shows broad-based budding of a yeast form, a major diagnostic feature. C) Lung showing B. dermatitidis yeast. D) Oral mucosa showing 2 yeast forms of B. dermatitidis closely associated with the mucosal surface. E) Transmission electron micrograph showing 3 yeast forms of B. dermatitidis in lung tissue. Note the thick cell walls and crescent shapes of the yeast (scale bar = 2 µm). Hematoxylin and eosin stain (A), Grocott methenamine silver stain (B and inset), and immunoalkaline phosphatase with antibody against B. dermatitidis and naphthol fast-red with hematoxylin counterstain (C, D). Original magnifications ×400 (A, B, D) and ×630 (Inset, C).