Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 17, Number 2—February 2011

Research

Possible Increased Pathogenicity of Pandemic (H1N1) 2009 Influenza Virus upon Reassortment

Eefje J.A. Schrauwen, Sander Herfst, Salin Chutinimitkul, Theo M. Bestebroer, Guus F. Rimmelzwaan, Albert D.M.E. Osterhaus, Thijs Kuiken, and Ron A.M. FouchierComments to Author 
Author affiliations: Author affiliation: National Influenza Centre and Erasmus Medical Center Department of Virology, Rotterdam, the Netherlands

Main Article

Figure 1

Replication of wild-type and reassortant pandemic (H1N1) 2009 viruses in MDCK cells. MDCK cells were injected in duplicate with 0.01 50% tissue culture infective dose (TCID50) per cell of each virus: black, wild-type pandemic (H1N1) 2009; red, reassortant pandemic (H1N1) 2009–seasonal influenza (H1N1) basic polymerase (PB) 2 acidic polymerase; blue, reassortant pandemic (H1N1) 2009–seasonal influenza (H1N1) PB2; green, reassortant pandemic (H1N1) 2009–seasonal influenza (H3N2) PB1 neuraminidase

Figure 1. Replication of wild-type and reassortant pandemic (H1N1) 2009 viruses in MDCK cells. MDCK cells were injected in duplicate with 0.01 50% tissue culture infective dose (TCID50) per cell of each virus: black, wild-type pandemic (H1N1) 2009; red, reassortant pandemic (H1N1) 2009–seasonal influenza (H1N1) basic polymerase (PB) 2 acidic polymerase; blue, reassortant pandemic (H1N1) 2009–seasonal influenza (H1N1) PB2; green, reassortant pandemic (H1N1) 2009–seasonal influenza (H3N2) PB1 neuraminidase (NA); orange, reassortant pandemic (H1N1)–seasonal influenza (H3N2) NA. Supernatant samples were harvested 6, 12, 24 and 48 h after injection. Supernatant samples were titrated in MDCK cells. Geometric mean titers and standard deviation were calculated from 2 independent experiments.

Main Article

TOP