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Volume 17, Number 2—February 2011

Research

Possible Increased Pathogenicity of Pandemic (H1N1) 2009 Influenza Virus upon Reassortment

Eefje J.A. Schrauwen, Sander Herfst, Salin Chutinimitkul, Theo M. Bestebroer, Guus F. Rimmelzwaan, Albert D.M.E. Osterhaus, Thijs Kuiken, and Ron A.M. FouchierComments to Author 
Author affiliations: Author affiliation: National Influenza Centre and Erasmus Medical Center Department of Virology, Rotterdam, the Netherlands

Main Article

Table 2

Virus antigen expression and severity of lesion in different tissues of the lung of 3 ferrets inoculated with pandemic (H1N1) 2009 or reassortant pandemic (H1N1) 2009 viruses*

Virus
Cumulative score per tissue
Bronchial surface epithelium

Bronchial submucosal epithelium

Bronchiolar epithelium

Alveolar epithelium
IHC
H&E
IHC
H&E
IHC
H&E
IHC
H&E
Pandemic (H1N1) 2009 0, 0, 0† 0, 0, 0 0, 0, 1 0, 0, 1 0, 0, 1 0, 0, 1 0, 0, 0 0, 0, 0
Pandemic (H1N1) 2009–seasonal influenza A (H1N1) PB2PA 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0
Pandemic (H1N1) 2009–seasonal influenza A (H1N1) PB2 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0 0, 0, 0
Pandemic (H1N1) 2009–seasonal influenza A (H3N2) PB1NA 1, 1, 0 0, 1, 0 2, 1, 0 1, 1, 0 2, 1, 0 1, 1, 0 1, 1, 0 1, 1, 1
Pandemic (H1N1) 2009–seasonal influenza A (H3N2) NA 1, 1, 1 0, 1, 1 1, 0, 3 1, 0, 3 2, 2, 3 2, 2, 3 1, 0, 1 1, 1, 1

*IHC, immunohistochemistry to detected virus antigen expression; H&E, hematoxylin and eosin staining to analyze severity of inflammation; PB, basic polymerase; PA, acidic polymerase; NA, neuraminidase.
†Individual scores (as indicated in the Methods section) for 3 ferrets are listed.

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