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Volume 17, Number 3—March 2011

Research

Monitoring and Characterization of Oseltamivir-Resistant Pandemic (H1N1) 2009 Virus, Japan, 2009–2010

Makoto Ujike, Miho Ejima, Akane Anraku, Kozue Shimabukuro, Masatsugu Obuchi, Noriko Kishida, Xu Hong, Emi Takashita, Seiichiro Fujisaki, Kazuyo Yamashita, Hiroshi Horikawa, Yumiko Kato, Akio Oguchi, Nobuyuki Fujita, Masato Tashiro, Takato OdagiriComments to Author , and the Influenza Virus Surveillance Group of Japan
Author affiliations: Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (M. Ujike, M. Ejima, A. Anraku, K. Shimabukuro, M. Obuchi, N. Kishida, X. Hong, E. Takashita, S. Fujisaki, K. Yamashita, M. Tashiro, T. Odagiri); National Institute of Technology and Evaluation, Tokyo (H. Horikawa, Y. Kato, A. Oguchi, N. Fujita)

Main Article

Table 2

Amino acid differences of each internal protein between oseltamivir-resistant (n = 20) and oseltamivir-sensitive (n = 138) pandemic (H1N1) 2009 virus, by strain, Japan, 2009–2010*

Strain M1 M2 NP NS1 NS2 PA PB1† PB2†
A/Niigata/1459/2009 V122I I435V, N537S
A/Osaka/2024/2009 I435V R251K
A/Shimane/1062/2009 V127A, T357I I435V N448S
A/Shimane/188/2009 A102T I435V R54K
A/Yokohama/1340/2009 S186N I435V
A/Yokohama/1394/2009 I435V, F466Y
A/Shiga/61/2009 V119I M93I I435V, A93V, T257A K660R
A/Niigata/1233/2009 M93I, E217K T257A K660R
A/Niigata/1234/2009 M93I, E217K T257A K660R, D256G
A/Shiga/45/2009 K660R
A/Chiba/1017/2009
A/Iwate/3/2009 K103R‡
A/Mie/100/2009 S23N T94N V609A R251K
A/Oita/126/2009 I100V E55G, V103I L370I K480R
A/Osaka/180/2009 A33T V103I E63K I667T V649I, E700K
A/Saitama-C/88/2009 E208K M50I A70V V227I
A/Sapporo-C/190/2009 D21G
A/Shiga/43/2009 A652T
A/Tokushima/2/2009 M311I
A/Yamaguchi/22/2009 V379I

*M, matrix protein; NP, nucleoprotein; NS, nonstructural protein; PA, polymerase A; PB, polymerase B.
†Of the 138 oseltamivir-sensitive pandemic (H1N1) 2009 virus samples, I435V and K660R (boldface) were observed from 32 and 12 isolates, respectively. These changes would sporadically occur in both pandemic (H1N1) 2009 isolate types.
‡Lys (K) at position 103 in M1 protein, consensus amino acid among the oseltamivir-resistant pandemic (H1N1) 2009 virus, was replaced with Arg (R).

Main Article

1Members of the Influenza Virus Surveillance Group of Japan are listed at the end of this article.

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