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Volume 17, Number 3—March 2011

Research

Novel Picornavirus in Turkey Poults with Hepatitis, California, USA

Kirsi S. Honkavuori, H. L. Shivaprasad, Thomas BrieseComments to Author , Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian Lipkin
Author affiliations: Author affiliations: Columbia University, New York, New York, USA (K.S. Honkavuori, T. Briese, C. Street, D.L. Hirschberg, W.I. Lipkin); California Animal Health and Food Safety Laboratory System, Tulare, California, USA (H.L. Shivaprasad); 454 Life Sciences, Branford, Connecticut, USA (S.K. Hutchison)

Main Article

Table 1

Real-time PCR measurement of viral sequences in organ samples from turkey poults with turkey viral hepatitis compared with controls, California, USA, 2008–2010*

Poult no. Age, d Organ Ct Virus copies†
Infected
2993A 25 Liver 17.12 5.66 × 107
2993B 25 Liver 17.02 6.22 × 107
2993C 25 Liver 26.98 9.0 × 103
2993D 25 Liver 17.93 2.8 × 107
0091.1 28 Liver 17.24 5.1 × 10
0091.2 28 Liver 17.69 3.43 × 107
0091.3 28 Liver 23.26 2.57 × 105
1813.1 26 Liver 23.48 2.12 × 105
1813.2 26 Liver 21.23 1.53 × 106
1813.3 26 Intestine 20.94 1.97 × 106
0690 30 Liver 34.92 8.98 × 10°
1999 29 Liver 28.44 8.42 × 103
Pancreas 25.97 7.32 × 104


Intestine
24.85
1.96 × 105
Control
1621.1 42 Liver >36‡ Negative
1621.2 42 Liver >36‡ Negative
1621.3 42 Liver >36‡ Negative

*Ct, cycle threshold.
†In 300 ng total RNA. Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
‡Ct >36 was rated as negative on the basis of the highest dilution of standard representing 5 copies.

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