Volume 17, Number 3—March 2011
Research
Novel Picornavirus in Turkey Poults with Hepatitis, California, USA
Kirsi S. Honkavuori, H. L. Shivaprasad, Thomas Briese
, Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian Lipkin
, Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian LipkinTable 1
Real-time PCR measurement of viral sequences in organ samples from turkey poults with turkey viral hepatitis compared with controls, California, USA, 2008–2010*
| Poult no. | Age, d | Organ | Ct | Virus copies† |
|---|---|---|---|---|
| Infected | ||||
| 2993A | 25 | Liver | 17.12 | 5.66 × 107 |
| 2993B | 25 | Liver | 17.02 | 6.22 × 107 |
| 2993C | 25 | Liver | 26.98 | 9.0 × 103 |
| 2993D | 25 | Liver | 17.93 | 2.8 × 107 |
| 0091.1 | 28 | Liver | 17.24 | 5.1 × 10 |
| 0091.2 | 28 | Liver | 17.69 | 3.43 × 107 |
| 0091.3 | 28 | Liver | 23.26 | 2.57 × 105 |
| 1813.1 | 26 | Liver | 23.48 | 2.12 × 105 |
| 1813.2 | 26 | Liver | 21.23 | 1.53 × 106 |
| 1813.3 | 26 | Intestine | 20.94 | 1.97 × 106 |
| 0690 | 30 | Liver | 34.92 | 8.98 × 10° |
| 1999 | 29 | Liver | 28.44 | 8.42 × 103 |
| Pancreas | 25.97 | 7.32 × 104 | ||
| Intestine |
24.85 |
1.96 × 105 |
||
| Control | ||||
| 1621.1 | 42 | Liver | >36‡ | Negative |
| 1621.2 | 42 | Liver | >36‡ | Negative |
| 1621.3 | 42 | Liver | >36‡ | Negative |
*Ct, cycle threshold.
†In 300 ng total RNA. Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
‡Ct >36 was rated as negative on the basis of the highest dilution of standard representing 5 copies.
