Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 17, Number 3—March 2011

Research

Novel Picornavirus in Turkey Poults with Hepatitis, California, USA

Kirsi S. Honkavuori, H. L. Shivaprasad, Thomas BrieseComments to Author , Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian Lipkin
Author affiliations: Author affiliations: Columbia University, New York, New York, USA (K.S. Honkavuori, T. Briese, C. Street, D.L. Hirschberg, W.I. Lipkin); California Animal Health and Food Safety Laboratory System, Tulare, California, USA (H.L. Shivaprasad); 454 Life Sciences, Branford, Connecticut, USA (S.K. Hutchison)

Main Article

Table 2

Real-time PCR measurement of viral sequences in samples from turkey poults with turkey viral hepatitis compared with controls, California, USA, 2008–2010*

Poult no. Age, d Sample Ct Virus copies†
Infected
2641.1 29 Cloacal swab 21.68 2.28 × 107
Bile 31.44 7.58 × 104
Serum 43.12 1.2 × 102
2641.2 29 Cloacal swab >44‡ Neg
Bile 32.5 4.04 × 104
Serum 38.04 1.67 × 103
2641.3 29 Cloacal swab >44‡ Neg
Bile 28.60 3.84 × 105
Serum 34.18 1.55 × 104
2641.4 29 Cloacal swab 30.6 1.21 × 105
Bile 27.72 6.48 × 105
Serum 41.06 3.06 × 102
2641.5 29 Cloacal swab 28.11 5.09 × 105
Bile 32.8 5.84 × 104
Serum 41.33 2.71 × 102
394.1 28 Cloacal swab >44‡ Neg
Serum 43.27 8.17 × 101
394.2 28 Cloacal swab 40.7 4.55 × 102
Serum >44‡ Neg
394.3 28 Cloacal swab >44‡ Neg
Serum >44‡ Neg
394.4 28 Cloacal swab 29.62 2.14 × 105
Serum >44‡ Neg
394.5 28 Serum >44‡ Neg
394.6 28 Serum >44‡ Neg
394.7 28 Serum >44‡ Neg
394.8 28 Serum >44‡ Neg
394. 9 28 Serum 31.07 9.34 × 104
3302.1 39 Serum 36.38 3.08 × 103
3302.2 39 Serum >44‡ Neg
3302.3 39 Serum >44‡ Neg
3302.5
39
Serum
>44‡
Neg
Control
2491.1 32 Cloacal swab >44‡ Neg
2491.2 32 Cloacal swab 25.67 2.2 × 106
2491.3 32 Cloacal swab >44‡ Neg
2491.4 32 Cloacal swab >44‡ Neg
2491.5 32 Cloacal swab >44‡ Neg
2491.6 32 Cloacal swab 34.74 1.11 × 104
407.1 39 Cloacal swab >44‡ Neg
407.2 39 Cloacal swab >44‡ Neg
407.3 39 Cloacal swab >44‡ Neg
407.4 39 Cloacal swab >44‡ Neg

*Ct, cycle threshold; neg, negative.
†In 300 ng total RNA. Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
‡Ct >44 was rated as negative on the basis of the highest dilution of standard representing 5 copies.

Main Article

TOP