Volume 17, Number 3—March 2011
Research
Novel Picornavirus in Turkey Poults with Hepatitis, California, USA
Kirsi S. Honkavuori, H. L. Shivaprasad, Thomas Briese
, Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian Lipkin
, Craig Street, David L. Hirschberg, Stephen K. Hutchison, and W. Ian LipkinTable 2
Real-time PCR measurement of viral sequences in samples from turkey poults with turkey viral hepatitis compared with controls, California, USA, 2008–2010*
| Poult no. | Age, d | Sample | Ct | Virus copies† |
|---|---|---|---|---|
| Infected | ||||
| 2641.1 | 29 | Cloacal swab | 21.68 | 2.28 × 107 |
| Bile | 31.44 | 7.58 × 104 | ||
| Serum | 43.12 | 1.2 × 102 | ||
| 2641.2 | 29 | Cloacal swab | >44‡ | Neg |
| Bile | 32.5 | 4.04 × 104 | ||
| Serum | 38.04 | 1.67 × 103 | ||
| 2641.3 | 29 | Cloacal swab | >44‡ | Neg |
| Bile | 28.60 | 3.84 × 105 | ||
| Serum | 34.18 | 1.55 × 104 | ||
| 2641.4 | 29 | Cloacal swab | 30.6 | 1.21 × 105 |
| Bile | 27.72 | 6.48 × 105 | ||
| Serum | 41.06 | 3.06 × 102 | ||
| 2641.5 | 29 | Cloacal swab | 28.11 | 5.09 × 105 |
| Bile | 32.8 | 5.84 × 104 | ||
| Serum | 41.33 | 2.71 × 102 | ||
| 394.1 | 28 | Cloacal swab | >44‡ | Neg |
| Serum | 43.27 | 8.17 × 101 | ||
| 394.2 | 28 | Cloacal swab | 40.7 | 4.55 × 102 |
| Serum | >44‡ | Neg | ||
| 394.3 | 28 | Cloacal swab | >44‡ | Neg |
| Serum | >44‡ | Neg | ||
| 394.4 | 28 | Cloacal swab | 29.62 | 2.14 × 105 |
| Serum | >44‡ | Neg | ||
| 394.5 | 28 | Serum | >44‡ | Neg |
| 394.6 | 28 | Serum | >44‡ | Neg |
| 394.7 | 28 | Serum | >44‡ | Neg |
| 394.8 | 28 | Serum | >44‡ | Neg |
| 394. 9 | 28 | Serum | 31.07 | 9.34 × 104 |
| 3302.1 | 39 | Serum | 36.38 | 3.08 × 103 |
| 3302.2 | 39 | Serum | >44‡ | Neg |
| 3302.3 | 39 | Serum | >44‡ | Neg |
| 3302.5 |
39 |
Serum |
>44‡ |
Neg |
| Control | ||||
| 2491.1 | 32 | Cloacal swab | >44‡ | Neg |
| 2491.2 | 32 | Cloacal swab | 25.67 | 2.2 × 106 |
| 2491.3 | 32 | Cloacal swab | >44‡ | Neg |
| 2491.4 | 32 | Cloacal swab | >44‡ | Neg |
| 2491.5 | 32 | Cloacal swab | >44‡ | Neg |
| 2491.6 | 32 | Cloacal swab | 34.74 | 1.11 × 104 |
| 407.1 | 39 | Cloacal swab | >44‡ | Neg |
| 407.2 | 39 | Cloacal swab | >44‡ | Neg |
| 407.3 | 39 | Cloacal swab | >44‡ | Neg |
| 407.4 | 39 | Cloacal swab | >44‡ | Neg |
*Ct, cycle threshold; neg, negative.
†In 300 ng total RNA. Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
‡Ct >44 was rated as negative on the basis of the highest dilution of standard representing 5 copies.
