TY - JOUR
AU - Cottell, Jennifer L.
AU - Webber, Mark A.
AU - Coldham, Nick G.
AU - Taylor, Dafydd L.
AU - Cerdeño-Tárraga, Anna M.
AU - Hauser, Heidi
AU - Thomson, Nicholas R.
AU - Woodward, Martin J.
AU - Piddock, Laura J.V.
T1 - Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14
T2 - Emerging Infectious Disease journal
PY - 2011
VL - 17
IS - 4
SP - 645
SN - 1080-6059
AB - Antimicrobial drug resistance is a global challenge for the 21st century with the emergence of resistant bacterial strains worldwide. Transferable resistance to β-lactam antimicrobial drugs, mediated by production of extended-spectrum β-lactamases (ESBLs), is of particular concern. In 2004, an ESBL-carrying IncK plasmid (pCT) was isolated from cattle in the United Kingdom. The sequence was a 93,629-bp plasmid encoding a single antimicrobial drug resistance gene, blaCTX-M-14. From this information, PCRs identifying novel features of pCT were designed and applied to isolates from several countries, showing that the plasmid has disseminated worldwide in bacteria from humans and animals. Complete DNA sequences can be used as a platform to develop rapid epidemiologic tools to identify and trace the spread of plasmids in clinically relevant pathogens, thus facilitating a better understanding of their distribution and ability to transfer between bacteria of humans and animals.
KW - Bacteria
KW - Escherichia coli
KW - antimicrobial drug resistance
KW - extended-spectrum beta-lactamase
KW - CTX-M
KW - plasmid
KW - epidemiology
KW - research
KW - United Kingdom
KW - Germany
KW - Spain
KW - Australia
KW - China
DO - 10.3201/eid1704.101009
UR - https://wwwnc.cdc.gov/eid/article/17/4/10-1009_article
ER - End of Reference