TY - JOUR AU - Cottell, Jennifer L. AU - Webber, Mark A. AU - Coldham, Nick G. AU - Taylor, Dafydd L. AU - Cerdeño-Tárraga, Anna M. AU - Hauser, Heidi AU - Thomson, Nicholas R. AU - Woodward, Martin J. AU - Piddock, Laura J.V. T1 - Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14 T2 - Emerging Infectious Disease journal PY - 2011 VL - 17 IS - 4 SP - 645 SN - 1080-6059 AB - Antimicrobial drug resistance is a global challenge for the 21st century with the emergence of resistant bacterial strains worldwide. Transferable resistance to β-lactam antimicrobial drugs, mediated by production of extended-spectrum β-lactamases (ESBLs), is of particular concern. In 2004, an ESBL-carrying IncK plasmid (pCT) was isolated from cattle in the United Kingdom. The sequence was a 93,629-bp plasmid encoding a single antimicrobial drug resistance gene, blaCTX-M-14. From this information, PCRs identifying novel features of pCT were designed and applied to isolates from several countries, showing that the plasmid has disseminated worldwide in bacteria from humans and animals. Complete DNA sequences can be used as a platform to develop rapid epidemiologic tools to identify and trace the spread of plasmids in clinically relevant pathogens, thus facilitating a better understanding of their distribution and ability to transfer between bacteria of humans and animals. KW - Bacteria KW - Escherichia coli KW - antimicrobial drug resistance KW - extended-spectrum beta-lactamase KW - CTX-M KW - plasmid KW - epidemiology KW - research KW - United Kingdom KW - Germany KW - Spain KW - Australia KW - China DO - 10.3201/eid1704.101009 UR - https://wwwnc.cdc.gov/eid/article/17/4/10-1009_article ER - End of Reference