Volume 17, Number 4—April 2011
Research
Genome Sequence of SG33 Strain and Recombination between Wild-Type and Vaccine Myxoma Viruses
Christelle Camus-Bouclainville
, Magalie Gretillat, Robert Py, Jacqueline Gelfi, Jean-Luc Guérin, and Stéphane Bertagnoli
, Magalie Gretillat, Robert Py, Jacqueline Gelfi, Jean-Luc Guérin, and Stéphane BertagnoliTable
Myxoma virus genes with amino acids discrepancies between Lausanne and SG33 sequences*
| ORF | Position in genome† |
Nucleotide changes or % identity‡ | Amino acid changes or % identity§ | |
|---|---|---|---|---|
| Lausanne (18) | SG33 | |||
| M005L | 6383–4935 | 6369–4921 | A6351G | Silent |
| C6286 | R33Q | |||
| M006L | 7948–6422 | 7934–6408 | G6683A | Silent |
| T6608G | E447D | |||
| M011L¶ | 14125–13628 | |||
| M011bL | 14110–14012 | Initiates at M52 | ||
| A13890G | V28A | |||
| A13857C | V39G | |||
| M011aL | 14569–14126 | G14103A | A8V | |
| G14024T | C34Stop | |||
| M020L# | 20531–19197 | 20518–19181 | 20379 GAG insertion | Addition of L (aa 52) |
| M030L | 30037–29372 | 30024–29359 | T30011C | T10A |
| M031R | 30138–31316 | 30125–31303 | C30614T | A159V |
| M034L | 36864–33847 | 36851–33834 | T36186C | Y227C |
| M044R | 44157–46190 | 44144–46177 | A44593T | T146S |
| A44596G | N147D | |||
| G44940T | K261N | |||
| M047R | 48288–48962 | 48275–48949 | A48780G | T164A |
| M049R | 49312–50604 | 49299–50591 | G49777A | M155I |
| M053R | 52380–53159 | 52367–53146 | G53113A | D245N |
| M054R | 53183–54178 | 53170–54165 | 97% | 7 substitutions, 97% |
| M058R | 56201–56953 | 56188–56940 | C56404T | A68V |
| M062R | 58406–58879 | 58393–58866 | T58642C | I79T |
| M064R | 59631–60239 | 59617–60222 | 60131 AGA insertion | Addition of E (aa 163) |
| M069L# | 66614–66081 | 66598–66083 | 66101 T deletion | 6-aa addition |
| M073R | 70698–71279 | 70682–71263 | C70861T | A55V |
| M076R | 72702–75206 | 72686–75190 | 100% (72686–73782) 95% (73783–75190) | 11 substitutions, 98% |
| M077L | 75602–75174 | 75655–75158 | 91% | 8 substitutions, 94% identity |
| C75619A T75620C | Stop → C upstream of ATG, potential N-terminal 23 aa addition** | |||
| M078R | 75608–76327 | 75592–76311 | 94% | 11 substitutions, 95% |
| M079R | 76327–76980 | 76311–76964 | 96% | 4 substitutions, 98% |
| M080R | 77017–79374 | 77001–79358 | 95% (77001–77639) 99% (77640–79361) | 6 substitutions, 99% |
| M083L | 82636–81779 | 82605–81763 | 81958–81972 deletion | 218 YNVKA 222 deletion |
| M085R | 83302–84078 | 83271–84047 | C83976T | A225V |
| M092L | 91923–89965 | 91892–89934 | A90679C | S416A |
| M095L | 94089–92971 | 94058–92940 | A93326C A93328G | S255P |
| M096L | 96252–94120 | 96221–94089 | C95947T | A103T |
| M099L | 100099–97397 | 100068–97366 | A98212G | I630T |
| M111R | 106301–107593 | 106270–107562 | T107143G | V281G |
| M134R | 125694–131693 | 125663–131662 | G130985A | S1773N |
| M135R | 131699–132232 | 131668–132201 | 96% | 8 substitutions in second half of protein |
| M136R | 132368–132904 | 132387–132929 | 87% | KL insertion, 26 substitutions 83% |
| M137R | 132908–133837 | 132933–133862 | 85% | 48 substitutions, 84% |
| M138L | 134746–133877 | 134767–133898 | 84% | 53 substitutions, 81%†† |
| M139R | 134806–135369 | 134818–135381 | 91% | 13 substitutions, 93% |
| M140R | 135375–137033 | 135387–137045 | 90% | 51 substitutions, 90% |
| M141R | 137069–137722 | 137089–137757 | 80% | 53 substitutions, 9 insertions/ deletions, 76% |
| M142R | 137731–138648 | 137768–138697 | 89% | A306NITRI (C-terminal) 21 substitutions, 93% |
| M143R | 138665–139366 | 138701–139402 | 90% | 13 substitutions, 94% |
| M144R | 139411–140310 | 139452–140345 | 84% | 67 substitutions, EY deletion 77% |
| M146R | 140335–140658 | 140372–140695 | 86% | 15 substitutions, 85% |
| M147R | 140700–141563 | 140749–141609 | 84% | 31 substitutions, 89% |
| M148R | 141626–143650 | 141678–143799 | 75% | 217 substitutions, 67% |
| M149R | 143655–145124 | 143704–145173 | 85% | 63 substitutions, 87% ‡‡ |
| M150R | 145191–146672 | 145241–146713 | 83% | 96 substitutions, 80% ‡‡ |
| M151R | 146684–147682 | 85% identity on 467 bp | 84% identity on aa 1–157, C-terminal 176-aa deletion‡‡ | |
| M001R | 160190–160969 | 83% id on 244 bp | 87% identity on aa 181–260, N-terminal 180-aa deletion | |
| M151R-M001R§§ | 146732–147439 | |||
*Boldface indicates genes at the border of SG33 deletion. ORF, open reading frame.
†Stop codon not included.
‡Nucleotide changes with position in Lausanne genome, or identity percentage if too many.
§Amino acid changes with position in Lausanne ORF, or no. changes and identity percentage if too many.
¶ Apparition of a stop codon, leading to the potential translation of 2 polypeptides (M011aL and M011bL) from SG33 transcript.
# Identical to 6918 strain (21).
** Addition not probable in view of promoting region position.
††100% identity to 1,189 bp of MSD strain (17).
‡‡99.6% identity on 1,737 bp with MSW strain (discontinued sequences) (22).
§§ Fusion of 2 partial ORFs as a result of deletion.
