Figure 2. Western blot analysis of 10 μL of bacterial suspension (1.8 × 1010 CFU/mL) loaded to a gel and subjected to electrophoresis. The proteins were transferred onto a nitrocellulose membrane, which was incubated in mouse or human serum as described in Materials and Methods. Serum samples used were convalescent-phase serum of the Bordetella petrii–infected patient (A), a pool of serum specimens from B. pertussis–infected patients (B), and a pool of serum specimens from B. bronchiseptica–infected patients (C). Lane 1, B. holmesii; lane 2, B. petrii FR3799; lane 3, B. petrii FR3891; lane 4, B. petrii FR3996; lane 5, B. petrii FR3497; lane 6, B. petrii KMBW; lane 7, B. pertussis 8132; lane 8, B. parapertussis 12822; lane 9, B. bronchiseptica RB50. Arrows indicate the proteins specifically recognized by the anti-serum.