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Volume 17, Number 4—April 2011

Research

Bordetella petrii Infection with Long-lasting Persistence in Human

Alain Le Coustumier, Elisabeth Njamkepo, Vincent Cattoir, Sophie Guillot, and Nicole GuisoComments to Author 
Author affiliations: Author affiliations: Centre Hospitalier, Cahors, France (A. Le Coustumier); Institut Pasteur, Paris, France (E. Njamkepo, S. Guillot, N. Guiso); Centre Hospitalier Universitaire, Côte de Nacre, Caen, France (V. Cattoir)

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Figure 2

Western blot analysis of 10 μL of bacterial suspension (1.8 × 1010 CFU/mL) loaded to a gel and subjected to electrophoresis. The proteins were transferred onto a nitrocellulose membrane, which was incubated in mouse or human serum as described in Materials and Methods. Serum samples used were convalescent-phase serum of the Bordetella petrii–infected patient (A), a pool of serum specimens from B. pertussis–infected patients (B), and a pool of serum specimens from B. bronchiseptica–infected patie

Figure 2. Western blot analysis of 10 μL of bacterial suspension (1.8 × 1010 CFU/mL) loaded to a gel and subjected to electrophoresis. The proteins were transferred onto a nitrocellulose membrane, which was incubated in mouse or human serum as described in Materials and Methods. Serum samples used were convalescent-phase serum of the Bordetella petrii–infected patient (A), a pool of serum specimens from B. pertussis–infected patients (B), and a pool of serum specimens from B. bronchiseptica–infected patients (C). Lane 1, B. holmesii; lane 2, B. petrii FR3799; lane 3, B. petrii FR3891; lane 4, B. petrii FR3996; lane 5, B. petrii FR3497; lane 6, B. petrii KMBW; lane 7, B. pertussis 8132; lane 8, B. parapertussis 12822; lane 9, B. bronchiseptica RB50. Arrows indicate the proteins specifically recognized by the anti-serum.

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