Volume 17, Number 4—April 2011
Research
Molecular Epidemiology of Coxiella burnetii from Ruminants in Q Fever Outbreak, the Netherlands
Hendrik I.J. Roest
, Robin C. Ruuls, Jeroen J.H.C. Tilburg, Marrigje H. Nabuurs-Franssen, Corné H.W. Klaassen, Piet Vellema, René van den Brom, Daan Dercksen, Willem Wouda, Marcel A.H. Spierenburg, Arco N. van der Spek, Rob Buijs, Albert G. de Boer, Peter Th.J. Willemsen, and Fred G. van Zijderveld
, Robin C. Ruuls, Jeroen J.H.C. Tilburg, Marrigje H. Nabuurs-Franssen, Corné H.W. Klaassen, Piet Vellema, René van den Brom, Daan Dercksen, Willem Wouda, Marcel A.H. Spierenburg, Arco N. van der Spek, Rob Buijs, Albert G. de Boer, Peter Th.J. Willemsen, and Fred G. van ZijderveldTable 1
Overview of Coxiella burnetii genotyping results for farms sampled during human Q fever outbreak, the Netherlands, 2007–2010*
| Farm ID | Animal species | Approximate herd size | Year of sampling | Approximate abortions in year of sampling, % | Sample type | No. samples tested | No. samples included in study | MLVA typing results |
|
|---|---|---|---|---|---|---|---|---|---|
| MLVA ID | No. samples | ||||||||
| A | Dairy goats | 617 | 2008 | 25 | Vaginal swabs | 20 | 9 | CbNL01 | 7 |
| CbNL05 | 1 | ||||||||
| CbNL07 |
1 |
||||||||
| B |
Dairy goats |
598 |
2008 |
20 |
Vaginal swabs |
20 |
5 |
CbNL01 |
5 |
| C |
Dairy goats |
546 |
2008 |
25 |
Vaginal swabs |
20 |
20 |
CbNL01 |
20 |
| D | Dairy goats | 1,498 | 2008 | 19 | Vaginal swabs | 39 | 7 | CbNL01 | 6 |
| CbNL04 |
1 |
||||||||
| E | Dairy goats | 1,568 | 2008 | 8 (2007) | Fetal tissue | 3 | 3 | CbNL01 | 1 |
| CbNL09 | 1 | ||||||||
| CbNL11 |
1 |
||||||||
| H | Dairy goats | 606 | 2008 | 80 | Vaginal swabs | 13 | 8 | CbNL01 | 7 |
| CbNL02 |
1 |
||||||||
| J | Dairy goats | 459 | 2008 | None | Vaginal swabs | 3 | 3 | CbNL01 | 2 |
| CbNL08 |
1 |
||||||||
| M |
Dairy goats |
769 |
2008 |
None |
Vaginal swabs |
2 |
1 |
CbNL10 |
1 |
| N | Dairy goats | 1,187 | 2009 | 25 | Vaginal swabs | 20 | 20 | CbNL01 | 20 |
| Placenta |
1 |
1 |
CbNL01 |
1 |
|||||
| O | Dairy goats | 83 | 2009 | 7 | Vaginal swabs | 40 | 16 | CbNL01 | 14 |
| CbNL03 | 1 | ||||||||
| CbNL06 | 1 | ||||||||
| Milk |
1 |
1 |
CbNL01 |
1 |
|||||
| P |
Dairy goats |
548 |
2009 |
10 |
Vaginal swabs |
20 |
6 |
CbNL01 |
6 |
| Q |
Dairy goats |
340 |
2009 |
10 |
Vaginal swabs |
25 |
19 |
CbNL01 |
19 |
| AE |
Dairy goats |
500 |
2007 |
>5 |
Placenta |
1 |
1 |
CbNL12 |
1 |
| AF |
Dairy goats |
2,000 |
2007 |
>5 |
Placenta |
1 |
1 |
CbNL01 |
1 |
| AG |
Dairy goats |
590 |
2001 |
>5 |
Paraffin-embedded placenta |
1 |
1 |
1 |
|
| R |
Dairy cattle |
70 |
2007 |
<5 |
Placenta |
1 |
1 |
CbNL13 |
1 |
| Y | Dairy sheep | 184 | 2010 | None | Vaginal swabs | 5 | 1 | CbNL10 | 1 |
| Bulk tank milk sample |
1 |
1 |
CbNL10 |
1 |
|||||
| Z | Sheep | 2 | 2009 | 50 | Placenta | 1 | 1 | CbNL01 | 1 |
*ID, identification; MLVA, multilocus variable-number tandem-repeat analysis.
