Emerging Infectious Disease ISSN: 1080-6059

Rapid Genotyping of Swine Influenza Viruses

Polly W.Y. Mak, Chloe K.S. Wong, Olive T.W. Li, Kwok Hung Chan, Chung Lam Cheung, Edward S. Ma, Richard J. Webby, Yi Guan, Joseph S. Malik Peiris, and Leo L.M. Poon

Author affiliations: Author affiliations: The University of Hong Kong, Hong Kong Special Administrative Region, People’s Republic of China (P.W.Y. Mak, C.K.S. Wong, O.T.W. Li, K.H. Chan, C.L. Cheung, E.S. Ma, Y. Guan, J.S.M. Peiris, L.L.M. Poon); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (R.J. Webby); Hong Kong University–Pasteur Research Centre, Hong Kong (J.S.M. Peiris)

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Figure

Genotyping of A) polymerase acidic protein, B) hemagglutinin, and C) neuraminidase segments of A/swine/Hong Kong/201/2010 influenza (H1N1) virus. Black line, amplification signal of SYBR green dye; blue line, amplification signal of cyanine 5 dye; green line, threshold level. The x-axis denotes the cycle number of a quantitative PCR assay, and the y-axis denotes the fluorescence intensity over the background.

Figure. Genotyping of A) polymerase acidic protein, B) hemagglutinin, and C) neuraminidase segments of A/swine/Hong Kong/201/2010 influenza (H1N1) virus. Black line, amplification signal of SYBR green dye; blue line, amplification signal of cyanine 5 dye; green line, threshold level. The x-axis denotes the cycle number of a quantitative PCR assay, and the y-axis denotes the fluorescence intensity over the background.

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