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Volume 17, Number 5—May 2011

Dispatch

Tick-Borne Encephalitis Virus, Kyrgyzstan

Benjamin J. BriggsComments to Author , Barry Atkinson, Donna M. Czechowski, Peter A. Larsen, Heather N. Meeks, Juan P. Carrera, Ryan M. Duplechin, Roger Hewson, Asankadyr T. Junushov, Olga N. Gavrilova, Irena Breininger, Carleton J. Phillips, Robert J. Baker, and John Hay
Author affiliations: Author affiliations: State University of New York, Buffalo, New York, USA (B.J. Briggs, D.M. Czechowski, J. Hay); Health Protection Agency, Porton Down, Salisbury, UK (B. Atkinson, R. Hewson); Texas Tech University, Lubbock, Texas, USA (P.A. Larsen, H.N. Meeks, J.P. Carrera, R.M. Duplechin, C.J. Phillips, R.J. Baker); National Academy of Sciences of the Kyrgyz Republic, Bishkek, Kyrgyz Republic (A.T. Junushov); Ministry of Healthcare of the Kyrgyz Republic, Bishkek (O.N. Gavrilova, I. Breininger)

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Table 2

Primers used to test rodents, insectivores, and ticks for tick-borne encephalitis virus, Kyrgyzstan, 2007 and 2009*

Primer Gene Sequence, 5′ → 3′ Reference
FSM-1 NS5 GAGGCTGAACAACTGCACGA (8)
FSM-2 NS5 GAACACGTCCATTCCTGATCT (8)
FSM-1i NS5 ACGGAACGTGACAAGGCTAG (8)
FSM-2i NS5 GCTTGTTACCATCTTTGGAG (8)
TBEV913F E TGCACACAYYTGGAAAACAGGGA (9)
TBEV1738R E TGGCCACTTTTCAGGTGGTACTTGGTTCC (9)
RH TBE forward E GGCAGCATTGTGACCTGTGT R. Hewson, unpub. data
RH TBE reverse E CGTGTCCTGTGGCTTTCTTTTT R. Hewson, unpub. data
RH TBE probe E 6FAM-AGGYGKCYTGTGAGGC-MGB NFQ R. Hewson, unpub. data

*TBEV, tick-borne encephalitis virus; NS, nonstructural protein; E, envelope.

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