Emerging Infectious Disease ISSN: 1080-6059

Detection and Phylogenetic Characterization of Human Hepatitis E Virus Strains, Czech Republic

Petra Vasickova

, Michal Slany, Pavel Chalupa, Michal Holub, Radek Svoboda, and Ivo Pavlik

Author affiliations: Author affiliations: Veterinary Research Institute, Brno, Czech Republic (P. Vasickova, M. Slany, I. Pavlik); Charles University in Prague and University Hospital Bulovka, Prague, Czech Republic (P. Chalupa, M. Holub); Faculty of Medicine Masaryk University and the Faculty Hospital Brno, Brno (R. Svoboda)

Main Article

Figure

Phylogenetic tree constructed with MEGA version 3.1 software (www.megasoftware.net) by using the neighbor-joining method with 1,000 replication in bootstrap test based on 242-bp–long sequences within open reading frame 1 (OFR1) of hepatitis E virus (HEV) isolates and only bootstrap values (percentages) >50 are indicated on the tree. Key: ■, sequences originating from 5 Czech patients; □, representatives of genotype 3 subtypes: 3e (strain G2), 3f (strain G1), and 3g (strain Osh 205); and the 5

Figure. Phylogenetic tree constructed with MEGA version 3.1 software (www.megasoftware.net) by using the neighbor-joining method with 1,000 replication in bootstrap test based on 242-bp–long sequences within open reading frame 1 (OFR1) of hepatitis E virus (HEV) isolates and only bootstrap values (percentages) >50 are indicated on the tree. Key: ■, sequences originating from 5 Czech patients; □, representatives of genotype 3 subtypes: 3e (strain G2), 3f (strain G1), and 3g (strain Osh 205); and the 5 HEV strains most similar to human isolates from the Czech Republic: human strains from Germany, V0713286 and V0714229; swine strains from Japan, swJ12–4, swJ8–5, and swJB-E10; human strain from Japan, JNH-Ehi04L; swine strain from Hungary, HUN-072; swine strains from the Netherlands, NLSW28 and NLSW82; swine strain from Spain, SWP6; human strain from Spain, VH2; ▲, strains from the Czech Republic originating from domestic pigs, CZswHEV6 and CZswHEV21; and from wild boars, CZwbHEV51–09 and CZwbHEV71–09. GenBank accession numbers of chosen sequences are included in the phylogenetic tree. Scale bar indicates nucleotide substitutions per site.

Main Article

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.