Detection and Phylogenetic Characterization of Human Hepatitis E Virus Strains, Czech Republic
Petra Vasickova , Michal Slany, Pavel Chalupa, Michal Holub, Radek Svoboda, and Ivo Pavlik
Author affiliations: Author affiliations: Veterinary Research Institute, Brno, Czech Republic (P. Vasickova, M. Slany, I. Pavlik); Charles University in Prague and University Hospital Bulovka, Prague, Czech Republic (P. Chalupa, M. Holub); Faculty of Medicine Masaryk University and the Faculty Hospital Brno, Brno (R. Svoboda)
Figure. Phylogenetic tree constructed with MEGA version 3.1 software (www.megasoftware.net) by using the neighbor-joining method with 1,000 replication in bootstrap test based on 242-bp–long sequences within open reading frame 1 (OFR1) of hepatitis E virus (HEV) isolates and only bootstrap values (percentages) >50 are indicated on the tree. Key: ■, sequences originating from 5 Czech patients; □, representatives of genotype 3 subtypes: 3e (strain G2), 3f (strain G1), and 3g (strain Osh 205); and the 5 HEV strains most similar to human isolates from the Czech Republic: human strains from Germany, V0713286 and V0714229; swine strains from Japan, swJ12–4, swJ8–5, and swJB-E10; human strain from Japan, JNH-Ehi04L; swine strain from Hungary, HUN-072; swine strains from the Netherlands, NLSW28 and NLSW82; swine strain from Spain, SWP6; human strain from Spain, VH2; ▲, strains from the Czech Republic originating from domestic pigs, CZswHEV6 and CZswHEV21; and from wild boars, CZwbHEV51–09 and CZwbHEV71–09. GenBank accession numbers of chosen sequences are included in the phylogenetic tree. Scale bar indicates nucleotide substitutions per site.
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