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Volume 17, Number 5—May 2011

Dispatch

Detection and Phylogenetic Characterization of Human Hepatitis E Virus Strains, Czech Republic

Petra VasickovaComments to Author , Michal Slany, Pavel Chalupa, Michal Holub, Radek Svoboda, and Ivo Pavlik
Author affiliations: Author affiliations: Veterinary Research Institute, Brno, Czech Republic (P. Vasickova, M. Slany, I. Pavlik); Charles University in Prague and University Hospital Bulovka, Prague, Czech Republic (P. Chalupa, M. Holub); Faculty of Medicine Masaryk University and the Faculty Hospital Brno, Brno (R. Svoboda)

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Table A1

Characterization of the 10 patients tested in the study and detection of HEV RNA by nested RT-PCR*

Patient no. Age, y/sex Town of origin Possible risk factors Bilirubin, μmol/L ALT, μkat/L AST, μkat/L GGT μkat/L ALP, μkat/L Systemic disease Hospital care, d Isolate name ORF1† ORF2/3
1‡ 62/M A NS 152.00 38.40 19.30 5.60 4.83 NS 13 CZhHEV107-09
2‡ 66/M B Ate homemade slaughter products; used same knife and chopping board for raw and cooked meat; ate undercooked meat in sushi bar 110.00 40.78 8.72 15.37 4.73 NS 11 CZhHEV108-09 + +
3‡ 62/M B Used same knife and chopping board for raw and cooked meat 614.00 25.22 28.78 4.98 5.77 DM, IHD 41 CZhHEV113-09 + +
4‡ 75/M B NS 203.00 20.24 28.57 2.68 3.35 NS 15 CZhHEV117-09 + +
5‡ 77/F B Ate pork 77.00 78.87 51.78 8.92 6.92 NS 10 CZhHEV114-09 +
6‡ 59/M B NS 297.00 88.44 45.57 4.52 2.50 CLL 10 ND + +
7‡ 59/M C Ate raw meat 14.50 8.99 5.52 1.96 2.24 NS 8 ND
65/F C NS 36.30 2.32 0.90 8.76 4.83 BC, CH 11 ND
27/F C Visited China 5.80 1.89 2.08 1.03 1.40 NS 7 ND
10§ 53/M C NS 113.10 19.48 20.43 20.52 10.49 SM 14 CZhHEV197-09

*HEV, hepatitis E virus; RT-PCR, reverse transcription–PCR; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, gama-glutamyltransferase; ALP, alkaline phosphatase; ORF1, primers targeting open reading frame 1 of HEV genome; ORF2/3, primers targeting overlapping part of HEV ORF2 and ORF3; NS, not shown; DM, diabetes mellitus; IHD, ischemic heart disease; CLL, chronic lymphatic leukemia; ND, not detected; BC, breast cancer; CH, chronic hepatopathy; SM, smoldering myeloma.
†Specific PCR product sequenced.
‡Detection of immunoglobulin (Ig) G antibodies against HEV by EIAgen HEV IgM kit (Adaltis, Rome, Italy), EIAgen HEV IgG Kit (Adaltis) and Recomblot HEV IgM/IgG (Microgen GmbH, Neured, Germany).
§Detection of IgM antibodies against HEV by HEV IgM ELISA 3.0 (MP Diagnostics, Illkirch Cedex, France) and HEV ELISA (MP Diagnostics).

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